bs-1650R-BF647 [Conjugated Primary Antibody]
P53(Ser392) Polyclonal Antibody, AbBy Fluor® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P53 Ser392

Modification Site: Ser392

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: KLH conjugated synthetic phosphopeptide derived from human p53 around the phosphorylation site of (Ser392)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Conjugation: AbBy Fluor® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li X et al. Cyanidin-3-O-glucoside Restores Spermatogenic Dysfunction in Cadmium-exposed Pubertal Mice via Histone Ubiquitination and Mitigating Oxidative Damage. Journal of Hazardous Materials,2019, 121706.Read more>>
  • Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
  • Jianfang Wang. et al. Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis. J AGR FOOD CHEM. 2024;72(22):1264112654Read more>>