bs-1646R-BF405 [Conjugated Primary Antibody]
ERK1(T202/Y204)+ERK2(T183/Y185) Antibody, AbBy Fluor® 405 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERK1 Thr202/Tyr204+ERK2 Thr183/Tyr185

Modification Site: Thr183/Tyr185

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55945595

Swiss Prot: P28482

Source: KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Conjugation: AbBy Fluor® 405

Excitation/ Emission: 401nm/421nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Jianping, et al. "Bone Marrow Mononuclear Cell Transplantation Promotes Therapeutic Angiogenesis via Upregulation of the VEGF-VEGFR2 Signaling Pathway in a Rat Model of Vascular Dementia." Behavioural Brain Research (2014).Read more>>
  • Hirata, Azumi, et al. "Homeobox family Hoxc localization during murine palate formation." Congenital Anomalies (2016).Read more>>
  • Iriyama, Noriyoshi, Yoshihiro Hatta, and Masami Takei. "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." Cancer Medicine (2016).Read more>>
  • Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234Read more>>
  • Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161. Read more>>
  • Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019.Read more>>
  • Hao Huang. et al. MAP4K4 is involved in the neuronal development of retinal photoreceptors. EXP EYE RES. 2023 Aug;233:109524Read more>>
VALIDATION IMAGES