DATASHEET
Host:
Rabbit
Target Protein:
CD146/MCAM
Immunogen Range:
201-300/646
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
4162
Swiss Prot:
P43121
Source:
KLH conjugated synthetic peptide derived from human CD146
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Ying, et al. "Exendin-4 promotes proliferation of adipose-derived stem cells through ERK and JNK signaling pathways." In Vitro Cellular & Developmental Biology-Animal (2016): 1-9.Read more>>
- Kanthilal, Manisha. Characterization of Molecular and Mechanical Phenotypes of Freshly Isolated Lipoaspirate Cells. Diss. Brown University, 2013.Read more>>
- Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
- Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
- Heriansyah T et al. Polysaccharide peptide (PsP) Ganoderma lucidum: a potential inducer for vascular repair in type 2 diabetes mellitus model. Vasc Health Risk Manag. 2019 Oct 3;15:419-427. Read more>>
- Gao Y et al. Induction of autophagy protects human dental pulp cells from lipopolysaccharide?induced pyroptotic cell death. Exp Ther Med. 2020 Mar;19(3):2202-2210.Read more>>
- Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8Read more>>
- Jianyun Cao. et al. Puerarin promotes the osteogenic differentiation of rat dental follicle cells by promoting the activation of the nitric oxide pathway. Tissue Cell. 2021 Dec;73:101601Read more>>
- Tino Stauber. et al. Extrinsic Macrophages Protect While Tendon Progenitors Degrade: Insights from a Tissue Engineered Model of Tendon Compartmental Crosstalk. 2021 Sep 08Read more>>
- Zhou Guanglei. et al. Cytotoxicity and cell migration evaluation of a strontium silicate-based root canal sealer on stem cells from rat apical papilla: an in vitro study. BMC ORAL HEALTH. 2024 Dec;24(1):1-1Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CD146/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD146/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Lane 1: U251 cell lysates; Lane 2: U-2os cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U2OS cell lysates; Lane 4: Human U251 cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.