bs-1615R [Primary Antibody]
Cathepsin D/CTSD Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cathepsin D/CTSD

Immunogen Range: 101-200/412


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1509

Swiss Prot: P07339

Source: KLH conjugated synthetic peptide derived from human Capsin D

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 11/38/45


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hossain, Shahdat, Hiroyuki Arai, and Osamu Shido. "Neuroprotective Effect of Madecassoside Evaluated Using Amyloid β1-42-Mediated in Vitro and in Vivo Alzheimer’s Disease Models." International Journal of Indigenous Medicinal Plants (2014).Read more>>
  • Liao, Peng, et al. "Organellar proteome analyses of ricin toxin-treated HeLa cells." Toxicology and industrial health (2014): 0748233714549066.Read more>>
  • ailey Balouch. et al. Human INCL fibroblasts display abnormal mitochondrial and lysosomal networks and heightened susceptibility to ROS-induced cell death. Plos One. 2021 Feb;16(2):e0239689Read more>>
VALIDATION IMAGES

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Cathepsin D/CTSD Polyclonal Antibody (bs-1615R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded human skin of vulva tissue labeled with Anti-Cathepsin D Polyclonal Antibody, Unconjugated (bs-1615R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining


Lane 1: Rat Liver tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with Cathepsin D Polyclonal Antibody, Unconjugated (bs-1615R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.