bs-1613R
[Primary Antibody]
FOX3/NeuN Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: FOX3/NeuN
Immunogen Range: 51-150/312
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 146713
Swiss Prot: A6NFN3
Source: KLH conjugated synthetic peptide derived from human NeuN
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 34
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yu, Lu, et al. "Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-κB and STAT3 in Transient Focal Stroke." PloS one 8.2 (2013): e55839.Read more>>
- Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro</i> and< i> vivo</i>." Neuroscience 192 (2011): 572-579.Read more>>
- Li, Hongwei, et al. "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." Brain Research (2013).Read more>>
- Jiang, Wang-Lin, et al. "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." European journal of pharmacology 640.1 (2010): 75-81.Read more>>
- Zhao, Wenjuan, et al. "Aging reduces glial uptake and promotes extracellular accumulation of Aβ from a lentiviral vector." Frontiers in Aging Neuroscience 6 (2014): 210.Read more>>
- Ridder, Kirsten, et al. "Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment." OncoImmunology (2015).Read more>>
- Fontes JD, Ramsey J, Polk JM, Koop A, Denisova JV, Belousov AB (2015) Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin. PLoS ONE 10(5): e0125395.Read more>>
- Wei, Gang, et al. "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." Journal of Alzheimer's Disease 33.3 (2013): 863-880.Read more>>
- Hu, Hai Xia, et al. "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Corrigendum in/mmr/12/3/3998." Molecular medicine reports 12.1 (2015): 1321-1327.Read more>>
- Kopec, Ashley M., et al. "Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue." Journal of Neuroscience Methods (2017).Read more>>
- Wang, Jun, et al. "Anti‐inflammatory and retinal protective effects of capsaicin on ischemia‐induced injuries through the release of endogenous somatostatin." Clinical and Experimental Pharmacology and Physiology (2017).Read more>>
- Song, Eric, et al. "Surface chemistry governs cellular tropism of nanoparticles in the brain." Nature Communications (2017).Read more>>
- Guo et al. Bone marrow-derived, neural-like cells have the characteristics of neurons to protect the peripheral nerve in microenvironment. (2015) Stem.Cells.In. 2015:941625Read more>>
- Chen YC et al. Indole Compound NC009-1 Augments APOE and TRKA in Alzheimer's Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. J Alzheimers Dis. 2019;67(2):737-756.Read more>>
- H Mu et al. HCMV-encoded IE2 induces anxiety-depression and cognitive impairment in UL122 genetically-modified mice. Int J Clin Exp Pathol 2019;12(11):4087-4095.Read more>>
- Shuhua Chenet al. Allopregnanolone Promotes Neuronal and Oligodendrocyte Differentiation In Vitro and In Vivo : Therapeutic Implication for Alzheimers Disease. Neurotherapeutics. 2020 Jul 6. doi: 10.1007/s13311-020-00874-x.Read more>>
- Dingkang Xu. et al. Sphingosine\1\phosphate receptor 3 is implicated in BBB injury via the CCL2\CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28Read more>>
- Hui Zhang. et al. MiR\34a\5p up\regulates the IL\1/COX2/PGE2 inflammation pathway and induces the release of CGRP via inhibition of SIRT1 in rat trigeminal ganglion neurons. Febs Open Bio. 2021 Jan;11(1):300-311Read more>>
- Junting Xiao. et al. Arsenite induces ferroptosis in the neuronal cells via activation of ferritinophagy. Food Chem Toxicol. 2021 Mar;:112114Read more>>
- Zhixiong Li. et al. Recovery of post-stroke cognitive and motor deficiencies by Shuxuening injection via regulating hippocampal BDNF-mediated Neurotrophin/Trk Signaling. Biomed Pharmacother. 2021 Sep;141:111828Read more>>
- ai Xue. et al. Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29. Anal Cell Pathol. 2021;2021:6622713Read more>>
- Qianru Yang. et al. Long-term in vivo two-photon imaging of the neuroinflammatory response to intracortical implants and micro-vessel disruptions in awake mice. Biomaterials. 2021 Sep;276:12106Read more>>
- Yu Hei. et al. Multifunctional Immunoliposomes Enhance the Immunotherapeutic Effects of PD-L1 Antibodies against Melanoma by Reprogramming Immunosuppressive Tumor Microenvironment. 2021 Dec 16Read more>>
- Derya Kaya. et al. Allopurinol attenuates repeated traumatic brain injury in old rats: A preliminary report. EXP NEUROL. 2022 Nov;357:114196Read more>>
- Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684Read more>>
- Tomruk Canberk. et al. Effects of apelin on neonatal brain neurogenesis in L-NAME-induced maternal preeclampsia. SCI REP-UK. 2024 Aug;14(1):1-17Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebral cortex; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NeuN Polyclonal Antibody, Unconjugated bs-1613R at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOX3/NeuN Polyclonal Antibody(bs-1613R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FOX3/NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FOX3/NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.