VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat lung carcinoma labeled with Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: 293T lysates; Lane 2: Jurkat lysates probed with Gamma-Adaptin Polyclonal Antibody, Unconjugated (bs-1600R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C