Host: Rabbit
Target Protein: VEGF-C
Immunogen Range: 321-415/415
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 22341
Swiss Prot: P97953
Source: KLH conjugated synthetic peptide derived from mouse VEGF-C
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 46
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Zhuo, Wei, et al. "The CXCL12?CCXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis."Clinical Cancer Research 18.19 (2012): 5387-5398.lRead more>>
- He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015).Read more>>
- Alunno, Alessia, et al. "Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögrens syndrome." Journal of Cellular and Molecular Medicine (2016).Read more>>
- Cialdai, Francesca, et al. "Modeled Microgravity Affects Fibroblast Functions Related to Wound Healing." Microgravity Science and Technology: 1-12.Read more>>
- Shin et al. Expression of vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) in the glial reaction elicited by human mesenchymal stem cell engraftment in the normal rat brain. (2015) J.Histochem.Cytochem. 63:170-80Read more>>
- Alunno et al. Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögren's syndrome. (2016) J.Cell.Mol.Med. 20:613-22Read more>>
- Ferrão JSP et al. Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization. Vet Med Sci. 2019 Feb 11. Read more>>
- Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 DecRead more>>
- Michiko Jo. et al. Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping. MICROCIRCULATION. 2022 Oct;:e12788Read more>>
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Thymus lysates; Lane 4: Rat Lymph node lysates; Lane 5: Rat Kidney lysates probed with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-C Polyclonal Antibody(bs-1586R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).