bs-1563R [Primary Antibody]
E.coli O157:H7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E.coli O157:H7

Clonality: Polyclonal

Isotype: IgG

Source: Ecoli O157;H7 protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This antibody reacts with Escherichia coli O157 (E.coli O157), Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)

Cross Reactive Species: Others
(E.coli O157:H7/EHEC)

Predicted Cross Reactive Species: Bacteria

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Ze-Zhong, et al. "Indirect immunofluorescence detection of E. coli O157: H7 with fluorescent silica Nanoparticles." Biosensors and Bioelectronics (2014).Read more>>
  • Xue, Haiyan, et al. "Rapid Immunochromatographic Assay for Escherichia coli O157: H7 in Bovine Milk Using IgY Labeled by Fe 3 O 4/Au Composite Nanoparticles." Food Science and Technology Research 22.1 (2016): 53-58.Read more>>
  • Ana Fernndez. et al. Specific and Simultaneous Detection of E. coli O157:H7 and Shiga-like Toxins Using a Label-Free Photonic Immunosensor. PHOTONICS-BASEL. 2024 Apr;11(4):374Read more>>
VALIDATION IMAGES

Antigen: bs-1563P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm.