bs-1559R
[Primary Antibody]
GLP-1R Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GLP-1R
Immunogen Range: 101-200/463
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 25051
Swiss Prot: P32301
Source: KLH conjugated synthetic peptide derived from rat GLP-1R
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 51
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhou, Hao, et al. "Exendin-4 protects ADMSCs from apoptosis induced by Hydrogen peroxide through PI3K/Akt-Sfrp2 pathways." Free Radical Biology and Medicine (2014).Read more>>
- Nagai, Takuo, et al. "Linagliptin Ameliorates Methylglyoxal-Induced Peritoneal Fibrosis in Mice." PLOS ONE 11.8 (2016): e0160993.Read more>>
- Kanda et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. (2018) BMC.Cancer. 18:657Read more>>
- "Wen Y et al. Inhibitory effects of glucagon-like peptide-1 receptor on epilepsy. Biochem Biophys Res Commun. 2019 Mar 26;511(1):79-86."Read more>>
- Kim SJ et al. DPP-4 inhibition enhanced renal tubular and myocardial GLP-1 receptor expression decreased in CKD with myocardial infarction.BMC Nephrol. 2019 Mar 1;20(1):75. Read more>>
- Shan Y et al. The glucagon-like peptide-1 receptor agonist reduces inflammation and blood-brain barrier breakdown in an astrocyte-dependent manner in experimental stroke. J Neuroinflammation. 2019 Nov 28;16(1):242.Read more>>
- Melek T et al. Ileal interposition improves metabolic syndrome parameters in a rat model of metabolic syndrome induced by monosodium glutamateLife Sci.2020 Dec 9;266:118846.Read more>>
- Shuangli Yang. et al. Glucagon-like peptide-1 alleviates diabetic kidney disease through activation of autophagy by regulating AMP-activated protein kinase-mammalian target of rapamycin pathway. Am J Physiol-Endoc M. 2020 Nov;319(6):E1019-E103Read more>>
- Liu Jinlei. et al. Emodin Attenuated the Kidney Damage of High-Fat-Diet Mice via the Upregulation of Glucagon-Like Peptide-1 Receptor. Biomed Res Int. 2021;2021:6662704Read more>>
- Haoqiang Zhang. et al. Glucagon-like peptide-1 attenuated carboxymethyl lysine induced neuronal apoptosis via peroxisome proliferation activated receptor-. Aging-Us. 2021 Jul 31; 13(14): 19013C19027Read more>>
- Yuping Chen. et al. The Herb Pair Radix Rehmanniae and Cornus Officinalis Attenuated Testicular Damage in Mice With Diabetes Mellitus Through Butyric Acid/Glucagon-Like Peptide-1/Glucagon-Like Peptide-1 Receptor Pathway Mediated by Gut Microbiota. Front Microbiol. 2022 Feb 22;13:831881Read more>>
- Zhan-zhao Liu. et al. Glucagon-like peptide-1 receptor activation by liraglutide promotes breast cancer through NOX4/ROS/VEGF pathway. Life Sci. 2022 Apr;294:12037Read more>>
- Hongzeng Li. et al. Eugenol alleviated nonalcoholic fatty liver disease in rat via a gut-brain-liver axis involving glucagon-like Peptide-1. ARCH BIOCHEM BIOPHYS. 2022 May;:109269Read more>>
- Wu Li. et al. GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathway. J CLIN LAB ANAL. 2022 Aug;:e24604Read more>>
- Qiaoji Tian. et al. Glucagon-Like Peptide-1 Receptor Mediates the Improvement in Glycolipid Metabolism Disorder via AKT and AMPK Signalling Pathways in L02 Cells with Insulin Resistance. J MOL STRUCT. 2022 Oct;:134328Read more>>
- Li Xu. et al. Oleanolic acid and moderate drinking increase the pancreatic GLP-1R expression of the -cell mass deficiency induced hyperglycemia. PEERJ. 2023 Jul;11:e15705Read more>>
- Qi Ruibing. et al. Liraglutide improved the reproductive function of obese mice by upregulating the testicular AC3/cAMP/PKA pathway. REPROD BIOL ENDOCRIN. 2024 Dec;22(1):1-1Read more>>
- Pengfei Lv. et al. Intestinal Perfusion of Unacylated Ghrelin Alleviated Metabolic Associated Fatty Liver Disease in Rat via a Central Glucagon-Like Peptide-1 Pathway. AM J PHYSIOL-GASTR L. 2024 Apr;(Articles in Press)Read more>>
- Shaobin Yang. et al. Neuronostatin regulates neuronal function and energetic metabolism in Alzheimer's disease in a GPR107-dependent manner. NEUROPHARMACOLOGY. 2024 Nov;258:11009Read more>>
- Shaobin Yang. et al. Tirzepatide shows neuroprotective effects via regulating brain glucose metabolism in APP/PS1 mice. PEPTIDES. 2024 Jul;:171271Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U-937 cells were stained with GLP-1R Polyclonal Antibody(bs-1559R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Pancreas lysates; Lane 2: Mouse Lung lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Liver tissue lysates( negative control) probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.