bs-1548R [Primary Antibody]
FOXO3A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FOXO3A

Immunogen Range: 581-673/673


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2309

Swiss Prot: O43524

Source: KLH conjugated synthetic peptide derived from human FOXO3A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 74


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Morales, María Gabriela, et al. "Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy via the Mas receptor." Disease Models and Mechanisms(2016): dmm-023390.Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Shang Y et al. Over\expressed MST1 impaired spatial memory via disturbing neural oscillation patterns in mice. Genes Brain Behav. 2020 Jul;19(6):e12678.Read more>>
  • Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212Read more>>
  • Orozco-Aguilar Josu. et al. Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic flux. BIOL RES. 2023 Dec;56(1):1-19Read more>>
  • F?nd?k Damla Gl. et al. Decreased growth differentiation factor 9, bone morphogenetic protein 15, and forkhead box O3a expressions in the ovary via ulipristal acetate. Revista da Associacao Medica Brasileira. 2023 Aug;69:e20230381Read more>>
  • Hong Zhao. et al. The anti-hyperplasia effect of polysaccharide from Prunella vulgaris L. on mammary gland hyperplasia in rats through regulation of the AKT-FOXO3a signaling pathway and intestinal flora. J SCI FOOD AGR. 2024 JuRead more>>
  • Ruixue Zhang. et al. The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia. J TRANSL MED. 2024; 22: 71Read more>>
VALIDATION IMAGES

L1 Rat brain lysate L2 human colon carcinoma 30ug, probed (bs-1548R) at 1:200 in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 74kDa.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human DU145 cell lysates probed with FOXO3A Polyclonal Antibody, Unconjugated (bs-1548R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.