Host: Rabbit
Target Protein: ZNT8
Immunogen Range: 101-200/369
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 169026
Swiss Prot: Q8IWU4
Source: KLH conjugated synthetic peptide derived from human ZNT8
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Human Raji lysates; Lane 2: Mouse spleen lysates probed with Rabbit Anti-ZNT8 Polyclonal Antibody, Unconjugated (bs-15382R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ZNT8 Polyclonal Antibody (bs-15382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates probed with ZNT8 Polyclonal Antibody, Unconjugated (bs-15382R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.