Host: Rabbit
Target Protein: GPCR EX33
Immunogen Range: 21-120/369
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 53831
Swiss Prot: Q9NQS5
Source: KLH conjugated synthetic peptide derived from human GPCR EX33 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Mouse
Rabbit
Predicted Cross Reactive Species:
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human glioma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Mouse Spinal Cord lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
U937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GPR84 Polyclonal Antibody(bs-15353R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).