bs-1533R [Primary Antibody]
COX4/COX IV-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: COX4/COX IV-1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1327

Source: KLH conjugated synthetic peptide derived from mouse COX IV-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced CYTOCHROME C and donates them to molecular oxygen, which is then reduced to water. It is composed of CYTOCHROME A and CYTOCHROME B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qiao Z et al. Casticin inhibits esophageal cancer cell proliferation and promotes apoptosis by regulating mitochondrial apoptotic and JNK signaling pathways.(2018)Naunyn Schmiedebergs Arch Pharmacol.Nov 17Read more>>
  • Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. Read more>>
  • Delong Wang. et al. Baoyuan Jiedu Decoction Alleviates Cancer-Induced Myotube Atrophy by Regulating Mitochondrial Dynamics Through p38 MAPK/PGC-1 Signaling Pathway. Front Oncol. 2020; 10: 523577Read more>>
  • Jie Gao. et al. Ferulic acid targets ACSL1 to ameliorate lipid metabolic disorders in db/db mice. J Funct Foods. 2022 Apr;91:105009Read more>>
  • Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078Read more>>
  • Wang Yu. et al. BI1 Activates Autophagy and Mediates TDP43 to Regulate ALS Pathogenesis. MOL NEUROBIOL. 2024 Jul;:1-43Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-COX4/COX IV-1 Polyoclonal Antibody, Unconjugated (bs-1533R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A431 cell lysates probed with COX4I1 Polyclonal Antibody, Unconjugated (bs-1533R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.