bs-15307R [Primary Antibody]
C9b/C9 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Complement component C9b

Immunogen Range: 251-350/559


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 735

Source: KLH conjugated synthetic peptide derived from human Complement component C9b

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

C9 is a plasma protein synthesized in the liver and monocytes consisting of a single polypeptide chain. C9 is a part of the membrane attack complex (MAC), an important component of the immune system. The MAC forms upon complement system activation by invading pathogenic bacteria and consists of the four major complement proteins: C5b, C6, C7 and C8. These complement proteins bind to the outer surface of the plasma membrane of the invading cell. C9 binds to the membrane associated C5b-8 protein, which leads to the circular polymerization of 12-18 C9 molecules. These polymerized C9 molecules form a ring structure in the membrane. Molecules can then diffuse freely through this transmembrane channel, causing cell lysis and destruction of the invading bacterial cell.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 32/61


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jennifer L. McRae. et al. Blockade of the G-CSF Receptor Is Protective in a Mouse Model of Renal IschemiaCReperfusion Injury. J Immunol. 2020 Sep;205(5):1433-144Read more>>
  • ongoni, Anjan K.. et al. A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemiaCreperfusion injury. Sci Rep-Uk. 2021 Nov;11(1):1-12Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C9b/C9 Polyclonal Antibody, Unconjugated (bs-15307R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Human Jurkat cell lysates; Lane 2: Human HL-60 cell lysates probed with Complement component C9b Polyclonal Antibody, Unconjugated (bs-15307R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.