bs-1523R
[Primary Antibody]
CD63/MLA1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD63/MLA1
Immunogen Range: 101-200/238
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 967
Swiss Prot: P08962
Source: KLH conjugated synthetic peptide derived from human CD63
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 26
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Son, Kyung Jin, et al. "Microfluidic compartments with sensing microbeads for dynamic monitoring of cytokine and exosome release from single cells."Analyst (2015).Read more>>
- Son, Kyung Jin, et al. "Microfluidic compartments with sensing microbeads for dynamic monitoring of cytokine and exosome release from single cells."Analyst (2015).Read more>>
- Sun, Weijia, et al. "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." Cell Discovery 2 (2016).Read more>>
- Marzesco, Anne-Marie, et al. "Highly potent intracellular membrane-associated Aβ seeds." Scientific Reports 6 (2016): 28125.Read more>>
- Sung MS et al. Single-Molecule Co-Immunoprecipitation Reveals Functional Inheritance of EGFRs in Extracellular Vesicles. (2018) Small,14(42):e1802358. Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Lorena del Pozo-Acebo. et al. Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy. Int J Mol Sci. 2021 Jan;22(3):1105Read more>>
- Jik-Han Jung. et al. Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma. Sci Rep-Uk. 2021; 11: 217Read more>>
- Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611C362Read more>>
- Li, Kanglu. et al. Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. J Nanobiotechnol. 2022 Dec;20(1):1-2Read more>>
- Yessenia L. Molina. et al. Rottlerin Stimulates Exosome/Microvesicle Release Via the Increase of Ceramide Levels Mediated by Ampk in an In Vitro Model of Intracellular Lipid Accumulation. BIOMEDICINES. 2022 Jun;10(6):1316Read more>>
- Hglund Nina. et al. Isolation of Extracellular Vesicles From the Bronchoalveolar Lavage Fluid of Healthy and Asthmatic Horses. FRONT VET SCI. 2022 Jun;0:853Read more>>
- Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586Read more>>
- Jingsong Chen. et al. Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress-Related Gene.. OXID MED CELL LONGEV. 2022 Sep;2022:9774570-977457Read more>>
- Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11Read more>>
- Naixin Ding. et al. Mechanism of Exosomal LncRNA PART1 in Esophageal Cancer Angiogenesis by Targeting miR-302a-3p/CDC25A Axis. TECHNOL CANCER RES T. ;()Read more>>
- Juan Yan. et al. Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels. SCI ADV. 2023 Jul;9(29)Read more>>
- Ting Hong. et al. Exosomal circBBS2 inhibits ferroptosis by targeting miR-494 to activate SLC7A11 signaling in ischemic stroke. FASEB J. 2023 Aug;37(9):e23152Read more>>
- in Ma. et al. Down-regulation of COL1A1 inhibits tumor-associated fibroblast activation and mediates matrix remodeling in the tumor microenvironment of breast cancer. OPEN LIFE SCI. 2023 Jan;18(1)Read more>>
- Xiaofeng Chen. et al. Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis. REGEN THER. 2024 Mar;25:344Read more>>
- Woojin Back. et al. Charge-Based Isolation of Extracellular Vesicles from Human Plasma. ACS OMEGA. 2024;XXXX(XXX):XXX-XXXRead more>>
- Yixuan Liang. et al. Exosomes-mediated transmission of standard bovine viral diarrhea strain OregonC24Va in bovine trophoblast cells. J REPROD IMMUNOL. 2024 Aug;164:104254Read more>>
- Naihan Yuan. et al. CREG1 promotes bovine placental trophoblast cells exosome release by targeting IGF2R and participates in regulating organoid differentiation via exosomes transport. INT J BIOL MACROMOL. 2024 Aug;274:133298Read more>>
- Mara ngeles vila-Glvez. et al. Loading milk exosomes with urolithins boosts their delivery to the brain: Comparing the activity of encapsulated vs. free urolithins in SH-SY5Y neuroblastoma cells. FOOD BIOSCI. 2024 Oct;61:104888Read more>>
VALIDATION IMAGES
HepG2 cells probed with CD63/MLA1 Polyclonal Antibody, Unconjugated (bs-1523R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: A431 lysates; Lane 2: HL-60 lysates; Lane 3: MCF-7 lysates; Lane 4: U97MG lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD63/MLA1 Polyclonal Antibody(bs-1523R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).