Host: Rabbit
Target Protein: CD33
Immunogen Range: 101-200/334
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12489
Swiss Prot: Q63994
Source: KLH conjugated synthetic peptide derived from mouse CD33
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 40/37
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Shan, Wulin, et al. "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." The International Journal of Biochemistry & Cell Biology (2015).Read more>>
- Iwami et al. Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics. (2015) Transplantatio. 99:668-77Read more>>
- Jiang et al. Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells. (2014) Cell.Death.Dis. 5:e1270Read more>>
- Katarzyna Sikorskaet al. The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid- Uptake by BV-2 Microglial Cells. Materials (Basel). 2020 Jul 20;13(14):3227.Read more>>
Mouse splenocytes probed with CD33 Polyclonal Antibody, Unconjugated (bs-1514R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCK-7 lysates probed with CD33 Polyclonal Antibody, Unconjugated (bs-1514R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C