bs-1508R [Primary Antibody]
Cathepsin L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cathepsin L

Immunogen Range: 71-170/334


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 13039

Swiss Prot: P06797

Source: KLH conjugated synthetic peptide derived from mouse capsin L1 proprotein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19/30/37


Cross Reactive Species: Human
Rat
Pig

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hernáez B, Guerra M, Salas ML, Andrés G (2016) African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog 12(4): e1005595. Read more>>
  • Mathew Suji Eapen. et al. The Pathophysiology of COVID-19 and SARS-CoV-2 Infection: Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections. Am J Physiol-Lung C. 2021 Jan 1; 320(1): L158CL163Read more>>
VALIDATION IMAGES

L1 rat liver, L2 rat brain lysates probed (bs-1508R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 37kDa.


Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-Cathepsin L Polyclonal Antibody (bs-1508R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Cathepsin L Polyclonal Antibody (bs-1508R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded rat skin labeled with Anti-Cathepsin L/CTSL Polyclonal Antibody (bs-1508R), Unconjugated followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cathepsin L Polyclonal Antibody, Unconjugated (bs-1508R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Human HUVEC cell Lysates; Lane 2: Human K562 cell Lysates; Lane 3: Human MCF-7 cell Lysates. Probed with Cathepsin L polyclonal Antibody, unconjugated (bs-1508R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-1508R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.