bs-1500R [Primary Antibody]
Cathepsin B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cathepsin B Heavy Chain

Immunogen Range: 251-339/339


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 13030

Swiss Prot: P10605

Source: KLH conjugated synthetic peptide derived from mouse Capsin B heavy chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 23/28/37


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, B., et al. "Autophagy activation aggravates neuronal injury in the hippocampus of vascular dementia rats." Neural Regeneration Research 9.13 (2014): 1288.Read more>>
  • Liru Li. et al. Rapamycin Pretreatment Alleviates Cerebral Ischemia/Reperfusion Injury in Dose-Response Manner Through Inhibition of the Autophagy and NFB Pathways in Rats:. Dose-Response. 2020;18(3)Read more>>
  • Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333Read more>>
  • Egidi Maren Juliane. et al. Anti-angiogenic therapy using the multi-tyrosine kinase inhibitor Regorafenib enhances tumor progression in a transgenic mouse model of ?-cell carcinogenesis. BRIT J CANCER. 2023 Aug;:1-13Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining


Mouse Spleen lysates probed with Cathepsin B Polyclonal Antibody, Unconjugated (bs-1500R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.