bs-1495R
[Primary Antibody]
Occludin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Occludin
Immunogen Range: 431-522/522
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 18260
Swiss Prot: Q61146
Source: KLH conjugated synthetic peptide derived from mouse Occludin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Pig
Predicted Cross Reactive Species:
Rat
Dog
Cow
Horse
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Pan, Li, et al. "Effects of Soybean Agglutinin on Mechanical Barrier Function and Tight Junction Protein Expression in Intestinal Epithelial Cells from Piglets." International Journal of Molecular Sciences 14.11 (2013): 21689-21704.Read more>>
- Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144.Read more>>
- Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926.Read more>>
- Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014).Read more>>
- Guo, Xiaoling, et al. "Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells." Experimental Eye Research (2016).Read more>>
- Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512.Read more>>
- Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299Read more>>
- Hayes CL.et al. Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis. (2018)Scientific Reports, 8(1).Read more>>
- Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.Read more>>
- Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044.Read more>>
- Weber B et al. Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes. PLoS One. 2019 Jul 5;14(7):e0219211. Read more>>
- Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
- Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene. 2020 Oct 30;759:144999.Read more>>
- Changsheng Xing. et al. Microbiota regulate innate immune signaling and protective immunity against cancer. Cell Host Microbe. 2021 ApRead more>>
- Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
- Yaln Budayc Azimet. et al. Investigation of the relationship between inflammation and microbiota in the intestinal tissue of female and male rats fed with fructose: Modulatory role of metformin. DARU. 2024 Jun;:1-21Read more>>
VALIDATION IMAGES
Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-1495R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-1495R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).