Host: Rabbit
Target Protein: CD80
Immunogen Range: 111-265/288
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 941
Swiss Prot: P33681
Source: KLH conjugated synthetic peptide derived from human CD80
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 32
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Jie Yu. et al. Synergistic enhancement of immunological responses triggered by hyperthermia sensitive Pt NPs via NIR laser to inhibit cancer relapse and metastasis. Bioact Mater. 2021 MayRead more>>
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Human A549 probed with Rabbit Anti-CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Sp2/0 cell lysates; Lane 3: Rat Thymus lysates; Lane 4: Human Raji cell lysates; Lane 5: Human K562 cell lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raji cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD80 Polyclonal Antibody(bs-1479R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).