bs-1469R
[Primary Antibody]
GRP75 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GRP75
Immunogen Range: 561-679/679
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3313
Swiss Prot: P38646
Source: KLH conjugated synthetic peptide derived from human GRP75
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Kitamura Nao. et al. Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells. J Biochem. 2021 JulRead more>>
- Yi Zhao. et al. Role of mitochondria-endoplasmic reticulum coupling in lycopene preventing DEHP-induced hepatotoxicity. Food Funct. 2021 SepRead more>>
- Junjun Peng. et al. Endoplasmic reticulum-mitochondria coupling attenuates vanadium-induced apoptosis via IP3R in duck renal tubular epithelial cells. J INORG BIOCHEM. J Inorg Biochem. 2022 Apr;:111809Read more>>
- Peng Junjun. et al. Mitochondria-associated endoplasmic reticulum membranes participate mitochondrial dysfunction and endoplasmic reticulum stress caused by copper in duck kidney. ENVIRON SCI POLLUT R. 2023 May;:1-12Read more>>
- Yiling Zhang. et al. Inhibition of calcium imbalance protects hepatocytes from vanadium exposure induced inflammation by mediating mitochondrial-associated endoplasmic reticulum membranes. POULTRY SCIENCE. 2023 Aug;:103013Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Rat Pancreas tissue lysates; Lane 8: Human K562 cell lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human Jurkat cell lysates; Lane 11: Human HeLa cell lysates; Lane 12: Human HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.