bs-1465R [Primary Antibody]
GRB2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GRB2

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2885

Swiss Prot: P62993

Source: KLH conjugated synthetic peptide derived from human GRB2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: ELISA(ELISA=1:5000-10000)
IHC-P(IHC-P=1:100-500)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Rabbit Anti-GRB2/ASH Polyclonal Antibody (bs-1465R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-GRB2/ASH Polyclonal Antibody (bs-1465R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRB2/ASH) Polyclonal Antibody, Unconjugated (bs-1465R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRB2) Polyclonal Antibody, Unconjugated (bs-1465R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRB2 Polyclonal Antibody, Unconjugated (bs-1465R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.