bs-1456R [Primary Antibody]
Adenosine A2a receptor Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: A2AR/Adenosine A2a receptor

Immunogen Range: 165-300/412


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 135

Swiss Prot: P29274

Source: KLH conjugated synthetic peptide derived from human A2a receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cao, Zhi-Ping, et al. "Effects of cordycepin on spontaneous alternation behavior and adenosine receptors expression in hippocampus." Physiology & Behavior (2017).Read more>>
  • Aleix Mart Naviaet al. Adenosine Receptors as Neuroinflammation Modulators: Role of A1 Agonists and A2A Antagonists. Cells. 2020 Jul 21;9(7):1739.Read more>>
  • Junnan Cai. et al. Shufeng Jiedu capsule inhibits inflammation and apoptosis by activating A2AAR and inhibiting NF-B to alleviate LPS-induced ALI. J ETHNOPHARMACOL. 2022 Nov;298:115661Read more>>
  • Xue-qi Liu. et al. Blocking ATP-P1Rs axis attenuate alcohol-related liver fibrosis. LIFE SCI. 2023 Jun;:121896Read more>>
  • Jiajun Chen. et al. Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism. J PHARM ANAL. 2023 AugRead more>>
  • Lv B.. et al. Epoxyeicosatrienoic Acids Attenuate LPS-Induced NIH/3T3 Cell Fibrosis through the A2AR and PI3K/Akt Signaling Pathways. B EXP BIOL MED+. 2024 Aug;:1-5Read more>>
VALIDATION IMAGES

Lane 1:rat heart lysates Lane 2: rat brain lysates probed with Anti A2AR/Adenosine A2a receptor Polyclonal Antibody, Unconjugated (bs-1456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 64kD. Observed band size: 64kD.


Formalin-fixed and paraffin embedded mouse brain labeled with Anti-A2AR/Adenosine A2a Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


25 ug total protein per lane of various lysates (see on figure) probed with Adenosine Receptor A2a polyclonal antibody, unconjugated (bs-1456R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.