bs-1451R [Primary Antibody]
PAFAH POLYCLONAL ANTIBODY
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PAFAH

Immunogen Range: 301-441/441


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7941

Swiss Prot: Q13093

Source: KLH conjugated synthetic peptide derived from human PAFAH2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Pig
Rabbit

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Gangqi, et al. "Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NF [kgr] B pathways." Scientific Reports 4 (2014).Read more>>
  • Han et al. Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways. (2015) Biol.Open. 4:661-5Read more>>
  • Wihastuti TA et al. Decreasing angiogenesis vasa vasorum through Lp-PLA2 and H2O2 inhibition by PSP from Ganoderma lucidum in atherosclerosis: in vivo diabetes mellitus type 2. J Basic Clin Physiol Pharmacol. 2020 Feb 7;30(6). Read more>>
  • Yangyang Tao. et al. Melatonin suppresses atherosclerosis by ferroptosis inhibition via activating NRF2 pathway. FASEB J. 2024 May;38(10):e23678Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat spleen labeled with Anti-PAFAH Polyclonal Antibody, Unconjugated(bs-1451R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin embedded Rabbit Cartoid labeled with Anti-PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated (bs-1451R) followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated (bs-1451R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.