Host: Rabbit
Target Protein: HIF2 alpha
Immunogen Range: 1-130/874
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 13819
Swiss Prot: P97481
Source: KLH conjugated synthetic peptide derived from mouse HIF2 Alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 96
Cross Reactive Species:
Human
Mouse
Rat
Sheep
Monkey
Predicted Cross Reactive Species:
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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- Li, Hao, et al. "Prognostic value of CD147 and HIF-2α expression in localized clear cell renal cell carcinoma." Int J Clin Exp Pathol 9.9 (2016): 9394-9400.Read more>>
- Thom et al. Measurements of CD34+/CD45-dim Stem Cells Predict Healing of Diabetic Neuropathic Wounds. (2016) Diabetes. 65:486-97Read more>>
- Lixin L et al. Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes. Acta Histochem. 2019 Jul;121(5):539-545. Read more>>
- Tang Q et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure micethrough targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. Read more>>
- He Y et al. Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep. Animals (Basel). 2019 Sep 11;9(9). pii: E673. Read more>>
- Xiong Y et al. Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirdsPLoS Genet.2020 Dec 28;16(12):e1009270.Read more>>
- Shiying Huang. et al. Jujube polysaccharides mitigated anemia in rats with chronic kidney disease: Regulation of short chain fatty acids release and erythropoietin production. J Funct Foods. 2021 Nov;86:104673Read more>>
- Yanyu He. et al. Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats. Animals-Basel. 2021 Oct;11(10):2798Read more>>
- Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2. Tissue Cell. 2021 Sep;:101646Read more>>
- Cui, Yan. et al. Effects of PHD and HSP90 on erythropoietin production in yak (Bos grunniens) renal interstitial fibroblast-like cells under hypoxia. J Mol Histol. 2022 Jan;:1-17Read more>>
- Haipiao Huang. et al. Jian-Pi-Yi-Shen formula ameliorates renal fibrosis-induced anemia in rats with chronic kidney disease. J ETHNOPHARMACOL. 2024 Dec;335:118607Read more>>
- Qingbo Zheng. et al. Genetic structure analysis of yak breeds and their response to adaptive evolution. GENOMICS. 2024 Sep;116:110933Read more>>
Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2α Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).