bs-1447R
[Primary Antibody]
HIF2 alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HIF2 alpha
Immunogen Range: 1-130/874
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 13819
Swiss Prot: P97481
Source: KLH conjugated synthetic peptide derived from mouse HIF2 Alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP3. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 96
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Xu, et al. "Oxidative stress induces the decline of brain EPO expression in aging rats." Experimental Gerontology (2016).Read more>>
- Li, Hao, et al. "Prognostic value of CD147 and HIF-2α expression in localized clear cell renal cell carcinoma." Int J Clin Exp Pathol 9.9 (2016): 9394-9400.Read more>>
- Thom et al. Measurements of CD34+/CD45-dim Stem Cells Predict Healing of Diabetic Neuropathic Wounds. (2016) Diabetes. 65:486-97Read more>>
- Lixin L et al. Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes. Acta Histochem. 2019 Jul;121(5):539-545. Read more>>
- Tang Q et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure micethrough targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. Read more>>
- He Y et al. Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep. Animals (Basel). 2019 Sep 11;9(9). pii: E673. Read more>>
- Xiong Y et al. Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirdsPLoS Genet.2020 Dec 28;16(12):e1009270.Read more>>
- Shiying Huang. et al. Jujube polysaccharides mitigated anemia in rats with chronic kidney disease: Regulation of short chain fatty acids release and erythropoietin production. J Funct Foods. 2021 Nov;86:104673Read more>>
- Yanyu He. et al. Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats. Animals-Basel. 2021 Oct;11(10):2798Read more>>
- Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2. Tissue Cell. 2021 Sep;:101646Read more>>
- Cui, Yan. et al. Effects of PHD and HSP90 on erythropoietin production in yak (Bos grunniens) renal interstitial fibroblast-like cells under hypoxia. J Mol Histol. 2022 Jan;:1-17Read more>>
- Haipiao Huang. et al. Jian-Pi-Yi-Shen formula ameliorates renal fibrosis-induced anemia in rats with chronic kidney disease. J ETHNOPHARMACOL. 2024 Dec;335:118607Read more>>
- Qingbo Zheng. et al. Genetic structure analysis of yak breeds and their response to adaptive evolution. GENOMICS. 2024 Sep;116:110933Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2α Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4℃,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with HIF 2 alpha polyclonal antibody, unconjugated (bs-1447R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.