bs-1444R-BF647 [Conjugated Primary Antibody]
TLR3 Polyclonal Antibody, AbBy Fluor® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TLR3

Immunogen Range: 801-905/905


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 142980

Swiss Prot: Q99MB1

Source: KLH conjugated synthetic peptide derived from mouse TLR3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response (By similarity).

Conjugation: AbBy Fluor® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 100


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhu, W., et al. "Overexpression of Toll?\Like Receptor 3 in Spleen is Associated with Experimental Arthritis in Rats."Scandinavian Journal of Immunology 76.3 (2012): 263-270..Read more>>
  • Wang, Shaolan, et al. "Xenobiotic receptor PXR regulates innate immunity via activation of NLRP3 inflammasome in vascular endothelial cells." Journal of Biological Chemistry (2014): jbc-M114.Read more>>
  • Zhu, Wenhua, et al. "Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis." Clinical Immunology (2016).Read more>>
  • Milara et al. Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist. (2015) Respir.Res. 16:12Read more>>
  • Zhao R et al. Activation of Toll-like receptor 3 promotes pathological corneal neovascularization by enhancement of SDF-1-mediated endothelial progenitor cell recruitment. (2018) Exp Eye Res. 178:177-185.Read more>>
  • Zou Xiong. et al. Toll-Like Receptors Serve as Biomarkers for Early Diagnosis and Prognosis Assessment of Kidney Renal Clear Cell Carcinoma by Influencing the Immune Microenvironment: Comprehensive Bioinformatics Analysis Combined With Experimental Validation. Front Mol Biosci. 2022 Jan;0:24Read more>>
  • Shasha Liu. et al. STAT3 regulates antiviral immunity by suppressing excessive interferon signaling. CELL REP. 2023 Jul;42:112806Read more>>
VALIDATION IMAGES