Host: Rabbit
Target Protein: MC1R
Immunogen Range: 271-317/317
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 17199
Swiss Prot: Q01727
Source: KLH conjugated synthetic peptide derived from mouse MC1R
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Bekeschus, Sander, et al. "Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants." Oxidative Medicine and Cellular Longevity 2017 (2017).Read more>>
- Hae-Young Kim. et al. Low-Temperature Argon Plasma Regulates Skin Moisturizing and Melanogenesis-Regulating Markers through Yes-Associated Protein. Int J Mol Sci. 2021 Jan;22(4):1895Read more>>
paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 35kD. Observed band size: 35kD.
293T cells probed with MC-1R/MC1 Receptor Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated (bs-1419R-A488) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).
Lane 1: MOLT-4 Cells; Probed with MC-1R/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.