Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-NFATC1/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse Hippocampal tissue labeled with Anti-NFATc1/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) followed by conjugation to the secondary antibody


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:400 overnight at 4°C, DAB staining.


Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFAT2 Polyclonal Antibody(bs-1417R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).