bs-1402R-Cy7 [Conjugated Primary Antibody]
SREBP-1/2 Polyclonal Antibody, Cy7 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SREBP-1/2

Immunogen Range: 301-450/1147


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6720, 6721

Source: KLH conjugated synthetic peptide derived from human SREBP-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. [SUBUNIT] Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus. Belongs to the SREBP family.

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54/126


Cross Reactive Species: Human
Mouse
Rat
Bovine

Predicted Cross Reactive Species: Sheep
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Xian, et al. "ROS-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation."Antioxidants and Redox Signaling ja (2015).Read more>>
  • Guo, Xiao-fei, et al. "fat-1 mice protect against high-fat plus high-sugar diets induced non-alcoholic fatty liver disease." Food & Function (2017).Read more>>
  • Ma et al. Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats. (2017) Sci.Rep. 7:44819Read more>>
  • Fu et al. Lack of ClC-2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease. (2018) Cell.Physiol.Biochem. 45:2187-2198Read more>>
  • Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29. Read more>>
  • Li X et al. Lysine enhances the stimulation of fatty acids on milk fat synthesis via the GPRC6A-PI3K-FABP5 signaling in bovine mammary epithelial cells. J Agric Food Chem. 2019 Jun 26;67(25):7005-7015. Read more>>
  • Zhao Z et al. Cholesterol attenuated the progression of DEN-induced hepatocellular carcinoma via inhibiting SCAP mediated fatty acid de novo synthesis. Biochem Biophys Res Commun. 2019 Feb 19;509(4):855-861.Read more>>
  • Sun W et al. Baicalein reduced hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin resistant mice. Food Funct. 2020 Jan 7.Read more>>
  • Yang X et al. miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc. J Cell Biochem. 2019 Nov 10.Read more>>
  • Li P et al. CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. J Agric Food Chem. 2019 Sep 18;67(37):10513-10520. Read more>>
  • Chuang Guoet al. Recombinant Human lactoferrin attenuates the progression of hepatosteatosis and hepatocellular death by regulating iron and lipid homeostasis in ob/ob mice. Food Funct. 2020 Aug 1;11(8):7183-7196.Read more>>
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Chengjian Zhou. et al. Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway. 2020 Nov 08Read more>>
  • Yuyan Gu. et al. Ferulic Acid Ameliorates Atherosclerotic Injury by Modulating Gut Microbiota and Lipid Metabolism. Front Pharmacol. 2021; 12: 621339Read more>>
  • Yunhong Ren. et al. Probiotic-fermented black tartary buckwheat alleviates hyperlipidemia and gut microbiota dysbiosis in rats fed with high-fat diet. 2021 May 05Read more>>
  • Flavia G De Carvalho. et al. Taurine upregulates insulin signaling and mitochondrial metabolism in vitro, but not in adipocytes of obese women. Nutrition. 2021 Jul;:11143Read more>>
  • Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153Read more>>
  • Jia Han. et al. Zonarol Protected Liver from Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in a Mouse Model. Nutrients. 2021 Oct;13(10):3455Read more>>
  • Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Chen Luyao. et al. Epicatechin gallate prevents the de novo synthesis of fatty acid and the migration of prostate cancer cells. Acta Bioch Bioph Sin. 2021 OcRead more>>
  • Nora Helmrich. et al. Pharmacological Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice. Cell Mol Gastroenter. 2021 DecRead more>>
  • Hu, Shuang. et al. Deletion of p38 attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16Read more>>
  • Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
  • Tiange Li. et al. Chitosan oligosaccharide attenuates hepatic steatosis in HepG2 cells via the activation of AMP-activated protein kinase. 2022 Feb 2Read more>>
  • Yue-Qiang Huang. et al. Di-2-ethylhexyl phthalate (DEHP) induced lipid metabolism disorder in liver via activating the LXR/SREBP-1c/PPAR_/_ and NF-_B signaling pathway. FOOD CHEM TOXICOL. 2022 Jul;165:113119Read more>>
  • Jing Fan. et al. Chitosan Oligosaccharide Inhibits the Synthesis of Milk Fat in Bovine Mammary Epithelial Cells through AMPK-Mediated Downstream Signaling Pathway. ANIMALS. 2022 Jan;12(13):1692Read more>>
  • Cao, Hui. et al. 14-3-3_ is essential for milk composition stimulated by Leu/IGF-1 via IGF1R signaling pathway in BMECs. IN VITRO CELL DEV-AN. 2022 Jun;:1-12Read more>>
  • Shuang-Feng Xu. et al. Astrocyte-specific loss of lactoferrin influences neuronal structure and function by interfering with cholesterol synthesis. GLIA. 2022 AugRead more>>
  • Xin Jin. et al. GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells. CELL BIOL INT. 2022 AugRead more>>
  • Yingjun Zhou. et al. Chrysin improves diabetic nephropathy by regulating the AMPK-mediated lipid metabolism in HFD/STZ-induced DN mice. J FOOD BIOCHEM. 2022 Aug;:e14379Read more>>
  • Ang Li. et al. Tartary Buckwheat (Fagopyrum tataricum) Ameliorates Lipid Metabolism Disorders and Gut Microbiota Dysbiosis in High-Fat Diet-Fed Mice. FOODS. 2022 Jan;11(19):3028Read more>>
  • Mu?oz, V. R.. et al. Short-term physical exercise controls age-related hyperinsulinemia and improves hepatic metabolism in aged rodents. J ENDOCRINOL INVEST. 2022 Nov;:1-13Read more>>
  • Jiamei Liu. et al. Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility. ANIMALS. 2022 Jan;12(23):3302Read more>>
  • Lin Zhu. et al. Berberine Ameliorates Abnormal Lipid Metabolism via the Adenosine MonophosphateCActivated Protein Kinase/Sirtuin 1 Pathway in Alcohol-Related Liver Disease. LAB INVEST. 2023 Jan;:100041Read more>>
  • Jos R. Pauli. et al. Exercise training restores weight gain and attenuates hepatic inflammation in a rat model of non-celiac gluten sensitivity. J CELL BIOCHEM. 2023 FebRead more>>
  • Lu-Chang Liang. et al. Caffeic acid phenethyl ester reverses doxorubicin resistance in breast cancer cells via lipid metabolism regulation at least partly by suppressing the Akt/mTOR/SREBP1 pathway. KAOHSIUNG J MED SCI. 2023 MaRead more>>
  • Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14Read more>>
  • Chunqiu Fang. et al. Tiaogan Jiejiu Tongluo Formula attenuated alcohol-induced chronic liver injury by regulating lipid metabolism in rats. J ETHNOPHARMACOL. 2023 Dec;317:116838Read more>>
  • Mengjie Liu. et al. Baicalein enhances immune response in TNBC by inhibiting leptin expression of adipocytes. CANCER SCI. 2023 JulRead more>>
  • aoming Tian. et al. Yellow leaf green tea modulates AMPK/ACC/SREBP1c signaling pathway and gut microbiota in high-fat diet-induced mice to alleviate obesity. J SCI FOOD AGR. 2024 FebRead more>>
  • Dongmei Qin. et al. Lupeol improves bile acid metabolism and metabolic dysfunction-associated steatotic liver disease in mice via FXR signaling pathway and gut-liver axis. BIOMED PHARMACOTHER. 2024 Aug;177:116942Read more>>
  • Yaning Biao. et al. Wulingsan Alleviates MAFLD by Activating Autophagy via Regulating the AMPK/mTOR/ULK1 Signaling Pathway. CAN J GASTROENTEROL. 2024 Jul;2024(1):9777866Read more>>
VALIDATION IMAGES