bs-13909R [Primary Antibody]
CHK2 (Thr26) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CHK2 (Thr26)

Modification Site: Thr26

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11200

Swiss Prot: O96017

Source: KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr26

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Phospho-CHEK2 (Thr26) Polyclonal Antibody, Unconjugated (bs-13909R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.