bs-13903R [Primary Antibody]
Chimaerin 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Chimaerin 2

Immunogen Range: 251-350/468


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1124

Swiss Prot: P52757

Source: KLH conjugated synthetic peptide derived from human Chimaerin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

The Chimaerin gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, an SH2 domain and a Rho-GAP domain. This protein has GTPase-activating protein activity which is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Increased expression of this gene is associated with lymphomas and decreased expression of this gene is associated with high-grade gliomas and breast tumors. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)

Predicted Molecular Weight: 54


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Chimaerin 2 polyclonal antibody, unconjugated (bs-13903R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.