bs-13830R [Primary Antibody]
CELF3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CELF3

Immunogen Range: 401-465/465


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11189

Swiss Prot: Q5SZQ8

Source: KLH conjugated synthetic peptide derived from human CELF3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xianmin Meng. et al. MicroRNA-34a and microRNA-146a target CELF3 and suppress the osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch. J Dent Sci. 2021 DecRead more>>
VALIDATION IMAGES

Lane 1: Hepg2 lysates; Lane 2: A549 lysates probed with CELF3 Polyclonal Antibody, Unconjugated (bs-13830R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates ; Lane 5: Human SH-SY5Y cell lysates probed with CELF3 Polyclonal Antibody, Unconjugated (bs-13830R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.