bs-1374R [Primary Antibody]
BMP4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BMP4

Immunogen Range: 251-350/408


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from rat BMP4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 13/45


Cross Reactive Species: Mouse
Sheep

Predicted Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Xiaoli, Yansong Zhao, and Xin Wang. "Umbilical cord blood cells regulate the differentiation of endogenous neural stem cells in hypoxic ischemic neonatal rats via the hedgehog signaling pathway." Brain Research (2014).Read more>>
  • Yamaji K et al. Maldevelopment of the submandibular gland in a mouse model of Apert syndrome.(2018) Dev Dyn.Sep 20;10(10).Read more>>
  • Handeng Lyu. et al. Niche-mediated repair of airways is directed in an occupant-dependent manner. CELL REP. 2022 Dec;41:111863Read more>>
  • Shen, Weiyun. et al. Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury. DIS MODEL MECH. 2023 Jan;16(1)Read more>>
  • Zheng Ming-Hui. et al. Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21Read more>>
  • Guanghua Xiong. et al. Subchronic co-exposure of polystyrene nanoplastics and 3-BHA significantly aggravated the reproductive toxicity of ovaries and uterus in female mice. ENVIRON POLLUT. 2024 May;:124101Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMP4 Polyclonal Antibody, Unconjugated (bs-1374R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.