bs-1371R
[Primary Antibody]
Snail1/2/3 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: SNAI1, SNAI2, SNAI3
Immunogen Range: 188-264/264
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6615;6591;333929
Swiss Prot: Q02085;P97469;Q9QY31
Source: KLH conjugated synthetic peptide derived from human Snail
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
The zinc finger transcription factor 'SNAIL' was first identified in Drosophila and, along with 'twist', a basic helix-loop-helix transcription factor, is indispensable for mesoderm formation. SNAIL is a repressor of mouse E-cadherin transcription, with expression of SNAIL inversely correlated with expression of E-cadherin. Abnormal expression of SNAIL could underlie the tumorigenic conversion of epithelia associated with loss of E-cadherin expression through screening mouse and human cell lines and by in situ hybridization of primary human tumors undergoing malignant progression.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 29
Cross Reactive Species:
Human
Rabbit
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hsu et al. Epidermal growth factor-induced cyclooxygenase-2 enhances head and neck squamous cell carcinoma metastasis through fibronectin up-regulation. (2015) Oncotarge. 6:1723-39Read more>>
- Jinquan Cai Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. (2013) Exp.Ther.Me. 6:1489-1493Read more>>
- Pan et al. MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (2017) Exp.Ther.Med. 14:4663-4670Read more>>
- Lin et al. Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition. (2016) Front.Pharmacol. 6:320Read more>>
- Chaudhury et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. (2016) Nat.Commun. 7:13362Read more>>
- Chaudhury et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. (2016) Nat.Commun. 7:13362Read more>>
- Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217Read more>>
- Li Z et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019 Apr 24;184:227-233.Read more>>
- Li Z et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019 Apr 24;184:227-233.Read more>>
- Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20.Read more>>
- Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
- Huang T et al. Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer. Front Oncol. 2020 Jan 29;10:19.Read more>>
- Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
- Xin Wang. et al. Multiple roles of FGF10 in the regulation of corneal endothelial wound healing. Exp Eye Res. 2021 Apr;205:108517Read more>>
- Jun Li. et al. EBV-miR-BART12 inhibits cell migration and proliferation by targeting Snail expression in EBV-associated gastric cancer. 2021 Mar 01Read more>>
- Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
- Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
- Dr. Jing Tian. et al. Downregulation of SOX21-AS1 alleviated cisplatin resistance in cervical cancer through Epithelial-messenchymal transition (EMT) inhibition. REJUV RES. 2022 Aug 3Read more>>
- Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
- Xin Wenhu. et al. CLCA2 overexpression suppresses epithelial-to-mesenchymal transition in cervical cancer cells through inactivation of ERK/JNK/p38-MAPK signaling pathways. BMC MOL CELL BIOL. 2022 Dec;23(1):1-1Read more>>
- Xiaomeng Wang. et al. A multifunctional black phosphorus-based adhesive patch intrinsically induces partial EMT for effective burn wound healing. BIOMATER SCI-UK. 2022 NovRead more>>
- Wenting Liu. et al. ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway. J OCUL PHARMACOL TH. 2022 Dec 21Read more>>
- Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12Read more>>
- Yingmin Liu. et al. LOXL2 promotes tumor proliferation and metastasis by FAK/Src signaling in esophageal squamous cell carcinoma. ELECTRON J BIOTECHN. 2023 ApRead more>>
- Fangyu Chai. et al. Identification of SLC2A3 as a prognostic indicator correlated with the NF-B/EMT axis and immune response in head and neck squamous cell carcinoma. CHANNELS. 2023;17(1):Article: 2208928Read more>>
- Jinhua Gu. et al. Deciphering the mechanism of Peptostreptococcus anaerobius-induced chemoresistance in colorectal cancer: the important roles of MDSC recruitment and EMT activation. FRONT IMMUNOL. 2023; 14: 1230681Read more>>
- Jie Chen. et al. Quercetin ameliorates liver fibrosis in Wilson disease and EMT involving suppression of the Hedgehog signaling pathway. ARAB J CHEM. 2024 Jan;17:105487Read more>>
- Du Xiaoyun. et al. Reduced Proliferative Capacity and Defense against Staphylococcus aureus in Human Nasal Mucosal Epithelium Lacking ZNF365. INT ARCH ALLERGY IMM. 2024 Feb;:1-14Read more>>
- Tian Jihua. et al. Effects of the S1P/S1PR1 Signaling Pathway on High Glucose-Induced NRK-52E Epithelial-Mesenchymal Transition Via Regulation of ROS/NLRP3. INFLAMMATION. 2024 Aug;:1-15Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human rectal cancer labeled with Anti-Snail/SNAI 1 Polyclonal Antibody, Unconjugated (bs-1371R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human pancreas carcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin-embedded human laryngocarcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated(bs-1371R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1/2/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 polyclonal antibody, unconjugated (bs-1371R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.