VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
Image by provided by the Independent Validation program, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:100 followed by conjugation to the secondary antibody and DAPI staining
Formalin-fixed and paraffin-embedded rat brain labeled with AIF1 Polyclonal Antibody at 1:200 dilution followed by incubation with secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated (bs-1363R) at 1:200 for 40 minutes at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) 1:200, 40 minutes at 37°C
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
BV-microglia stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. AIF1 expression (green) in activated BV-microglia is observed using confocal microscopy. The blue staining represents DAPI. (a) Control; (b) treated with CCL2 (3 ng/mL); (c) CCL2 (3 ng/mL) + l-CDL (3 µM); (d) CCL2 (3 ng/mL) + l-CDL (10 µM); (e) CCL2 (3 ng/mL) + l-CDL (30 µM); (f) CCL2 (3 ng/mL) + RS504393 (10 µM);
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.