bs-1363R [Primary Antibody]
AIF1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AIF1

Immunogen Range: 51-147/147


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 199

Swiss Prot: P55008

Source: KLH conjugated synthetic peptide derived from human AIF1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hsu, Shih-Tien, et al. "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." Scientific Reports 7 (2017): 42280.Read more>>
  • Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017).Read more>>
  • Song, Eric, et al. "Surface chemistry governs cellular tropism of nanoparticles in the brain." Nature Communications (2017).Read more>>
  • Hu. et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 PathwayRead more>>
  • Ko, Chien-Hsin, et al. "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." Scientific reports 7.1 (2017): 17489.Read more>>
  • Furukawa et al. Databases for technical aspects of immunohistochemistry. (2017) J.Toxicol.Patho. 30:79-107Read more>>
  • Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
  • Ji et al. TFP5 peptide, derived from CDK5-activating cofactor p35, provides neuroprotection in early-stage of adult ischemic stroke. (2017) Sci.Rep. 7:40013Read more>>
  • Yue J et al. Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex. Brain Dev. 2019 Sep 5. pii: S0387-7604(19)30240-2. Read more>>
  • Liao CF et al. Effects of endogenous inflammation signals elicited by nerve growth factor, interferon-γ, and interleukin-4 on peripheral nerve regeneration. J Biol Eng. 2019 Nov 13;13:86. Read more>>
  • Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
  • Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
  • Fange Kong. et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-B signaling in Alzheimers disease. J Neuroinflamm. 2020 Dec;17(1):1-15Read more>>
  • Dingkang Xu. et al. Sphingosine\1\phosphate receptor 3 is implicated in BBB injury via the CCL2\CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28Read more>>
  • Lushuang Xie. et al. Inflammatory factors and amyloid -induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538C22549Read more>>
  • Pengfei Fu. et al. Baicalein Ameliorates Epilepsy Symptoms in a Pilocarpine-Induced Rat Model by Regulation of IGF1R. Neurochem Res. 2020 Dec;45(12):3021-3033Read more>>
  • Yanan Shen. et al. Phosphatase and Tensin Homolog Deleted on Chromosome Ten Knockdown Attenuates Cognitive Deficits by Inhibiting Microglia-mediated Neuroinflammation in the Hippocampus in a Mouse Model of Perioperative Neurocognitive Disorder. Neuroscience. 2021 Aug;468:199Read more>>
  • Shingo MIYAZAKI. et al. Spontaneous adenocarcinoma with giant cell formation in the accessory sex glands in a male Sprague-Dawley rat. 2021 Jul 23Read more>>
  • Tong Wang. et al. Reactive astrocytes induced by 2-chloroethanol modulate microglia polarization through IL-1, TNF-, and iNOS upregulation. Food Chem Toxicol. 2021 Sep;:11255Read more>>
  • Meilan Xue. et al. Neuroprotective effect of fucoidan by regulating gut-microbiota-brain axis in alcohol withdrawal mice. J Funct Foods. 2021 Nov;86:104726Read more>>
  • Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493Read more>>
  • Haiyang Zhang. et al. Co-exposure of chronic stress and alumina nanoparticles aggravates hippocampal microglia pyroptosis by activating cathepsin B/NLRP3 signaling pathway. J HAZARD MATER. 2022 Aug;436:129093Read more>>
  • Takei, Yoshinori. et al. Alteration in peritoneal cells with the chemokine CX3CL1 reverses age-associated impairment of recognition memory. GEROSCIENCE. 2022 May;:1-14Read more>>
  • Wei, Ying. et al. Astrocyte Activation, but not Microglia, Is Associated with the Experimental Mouse Model of Schizophrenia Induced by Chronic Ketamine. J MOL NEUROSCI. 2022 Jul;:1-14Read more>>
  • Chuanjiang He. et al. MiRNA-494 specifically inhibits SIRT3-mediated microglia activation in sepsis-associated encephalopathy. TRANSL CANCER RES. 2022 Jul; 11(7): 22992309Read more>>
  • Qinrui Hu. et al. Neuroprotection of boropinol-B in Cerebral Ischemia-reperfusion Injury by Inhibiting Inflammation and Apoptosis. BRAIN RES. 2022 Oct;:148132Read more>>
  • Yuxin Liu. et al. Ferromagnetic flexible electronics for brain-wide selective neural recording. ADV MATER. 2022 Nov;:2208251Read more>>
  • Can Sezer. et al. Neuroprotective Effects of Milrinone on Acute Traumatic Brain ?njury. WORLD NEUROSURG. 2022 NovRead more>>
  • Wang Shang. et al. Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimers disease in APP/PS1 mouse. ALZHEIMERS RES THER. 2023 Dec;15(1):1-17Read more>>
  • Guang-Sheng Li. et al. Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous Functional Recovery in Experimental Cervical Spondylotic Myelopathy. INT J MOL SCI. 2023 Jan;24(4):3408Read more>>
  • Longcong Dong. et al. Shared Genes of PPARG and NOS2 in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation. INT J MOL SCI. 2023 Jan;24(6):5651Read more>>
  • Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:11664Read more>>
  • Quancheng Cheng. et al. Integrated multiomics analysis reveals changes in liver physiological function in Aqp9 gene knockout mice. INT J BIOL MACROMOL. 2023 Jun;:125459Read more>>
  • Linna Sun. et al. Construction of Curcumin-Loaded Hydrogels for Treatment of Traumatic Brain Injury. ACS APPL POLYM MATER. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Xue Zhao. et al. Neural Stem Cell-Derived Exosomes Improve Neurological Function in Rats with Cerebral Ischemia-Reperfusion Injury by Regulating Microglia-Mediated Inflammatory Response. J INFLAMM RES. 2023 Jul 23Read more>>
  • Tomruk Canberk. et al. Effects of apelin on neonatal brain neurogenesis in L-NAME-induced maternal preeclampsia. SCI REP-UK. 2024 Aug;14(1):1-17Read more>>
  • Cui Lianxu. et al. Exosomal miRNA-21 derived from umbilical cord mesenchymal stem cells inhibits microglial overactivation to counteract nerve damage. MOL BIOL REP. 2024 Dec;51(1):1-9Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat brain labeled with AIF1 Polyclonal Antibody at 1:200 dilution followed by incubation with secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated (bs-1363R) at 1:200 for 40 minutes at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) 1:200, 40 minutes at 37°C


Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


araformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


BV-microglia stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. AIF1 expression (green) in activated BV-microglia is observed using confocal microscopy. The blue staining represents DAPI. (a) Control; (b) treated with CCL2 (3 ng/mL); (c) CCL2 (3 ng/mL) + l-CDL (3 µM); (d) CCL2 (3 ng/mL) + l-CDL (10 µM); (e) CCL2 (3 ng/mL) + l-CDL (30 µM); (f) CCL2 (3 ng/mL) + RS504393 (10 µM);


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat small Intestine;; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AIF1 Polyclonal Antibody(bs-1363R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF1 (9A3) Polyclonal Antibody, Unconjugated(bs-1363R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-AIF1/Iba1 antibody (bs-1363R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.


4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (AIF1/Iba1) polyclonal Antibody, unconjugated (bs-1363R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


25 ug total protein per lane of various lysates (see on figure) probed with AIF1 (9A3) polyclonal antibody, unconjugated (bs-1363R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.