Host: Rabbit
Target Protein: GADD45
Immunogen Range: 65-165/165
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1647
Swiss Prot: P24522
Source: KLH conjugated synthetic peptide derived from human GADD45
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Conjugation: FITC
Excitation/ Emission: 494nm/518nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 18
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.Read more>>
- Barış et al. Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement?. (2015) Turk.J.Haematol. 32:295-303Read more>>
- Xin-Li Xiao. et al. Status epilepticus induced Gadd45b is required for augmented dentate neurogenesis. Stem Cell Res. 2020 Dec;49:102102Read more>>
- Qingfan Mo. et al. BTNL9 is frequently downregulated and inhibits proliferation and metastasis via the P53/CDC25C and P53/GADD45 pathways in breast cancer. Biochem Bioph Res Co. 2021 May;553:17Read more>>
- Li Liu. et al. Nanosilver inhibits the progression of pancreatic cancer by inducing a paraptosis-like mixed type of cell death. BIOMED PHARMACOTHER. 2022 Sep;153:113511Read more>>
- in Yan. et al. Tumor and stroma COL8A1 secretion induces autocrine and paracrine progression signaling in pancreatic ductal adenocarcinoma. MATRIX BIOL. 2022 NovRead more>>
- Ming Xie. et al. GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(14):11304Read more>>
A431 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained withGADD45 Polyclonal Antibody, FITC Conjugated (bs-1360R-FIT) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).