Host: Rabbit
Target Protein: GADD45
Immunogen Range: 65-165/165
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1647
Swiss Prot: P24522
Source: KLH conjugated synthetic peptide derived from human GADD45
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 18
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.Read more>>
- Barış et al. Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement?. (2015) Turk.J.Haematol. 32:295-303Read more>>
- Xin-Li Xiao. et al. Status epilepticus induced Gadd45b is required for augmented dentate neurogenesis. Stem Cell Res. 2020 Dec;49:102102Read more>>
- Qingfan Mo. et al. BTNL9 is frequently downregulated and inhibits proliferation and metastasis via the P53/CDC25C and P53/GADD45 pathways in breast cancer. Biochem Bioph Res Co. 2021 May;553:17Read more>>
- Li Liu. et al. Nanosilver inhibits the progression of pancreatic cancer by inducing a paraptosis-like mixed type of cell death. BIOMED PHARMACOTHER. 2022 Sep;153:113511Read more>>
- in Yan. et al. Tumor and stroma COL8A1 secretion induces autocrine and paracrine progression signaling in pancreatic ductal adenocarcinoma. MATRIX BIOL. 2022 NovRead more>>
- Ming Xie. et al. GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(14):11304Read more>>
Formalin-fixed and paraffin embedded rat heart labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Rabbit Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 18kD. Observed band size: 18kD.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.