bs-13507R [Primary Antibody]
GPR84 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPR84

Immunogen Range: 1-100/396


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 53831

Swiss Prot: Q9NQS5

Source: KLH conjugated synthetic peptide derived from human GPR84

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 84 (GPR84), a member of the GCPR 1 family, is an orphan GCPR expressed in bone marrow, brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta, intestine, lung and peripheral blood leukocytes. In activated T cells, GPR84 regulates early interleukin-4 (IL-4) gene expression.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 44


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Horse
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yongxiang Li. et al. Food reward depends on TLR4 activation in dopaminergic neurons. Pharmacol Res. 2021 Jul;169:105659Read more>>
  • Weijie Zhao. et al. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice. FOODS. 2023 Jan;12(2):357Read more>>
  • Jinyan Liu. et al. MDSCs-derived GPR84 induces CD8+ T-cell senescence via p53 activation to suppress the antitumor response. J IMMUNOTHER CANCER. 2023 Nov;11(11):e007802Read more>>
VALIDATION IMAGES

Lane 1: SP2/0 Cell lysates; Lane 2: Mouse lung lysates; Lane 3: HL-60 Cell lysates; Lane 4: Mouse muscle lysates; probed with GPR84 Polyclonal Antibody, unconjugated (bs-13507R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Bone lysates; Lane 4: Rat Lung lysates ; Lane 5: Rat Cerebrum lysates;Lane 6: Rat Cerebellum lysates; Lane 7: Human U937 cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human THP-1 lysates probed with GPR84 Polyclonal Antibody, Unconjugated (bs-13507R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.