Host: Rabbit
Target Protein: MBOAT4/Ghrelin O acyltransferase
Immunogen Range: 101-200/435
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 619373
Swiss Prot: Q96T53
Source: KLH conjugated synthetic peptide derived from human Ghrelin O acyltransferase
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 50
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Horse
Rabbit
Monkey
For research use only. Not intended for diagnostic or therapeutic use.
- Yu, Ching‐Han, et al. "Mediation of oxidative stress in hypothalamic ghrelin‐associated appetite control in rats treated with phenylpropanolamine." Genes, Brain and Behavior (2016).Read more>>
- Yu, Ching-Han, et al. "Participation of ghrelin signalling in the reciprocal regulation of hypothalamic NPY/POMC-mediated appetite control in amphetamine-treated rats." Appetite (2017).Read more>>
- Yuan Zhang. et al. The Ghrelin/Growth Hormone Secretagogue Receptor System Is Involved in the Rapid and Sustained Antidepressant-Like Effect of Paeoniflorin. Front Neurosci-Switz. 2021; 15: 631424Read more>>
Lane 1: Mouse stomach lysates probed with Ghrelin O acyltransferase Antibody, Unconjugated (bs-13355R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (bs-13355R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.