DATASHEET
Host:
Rabbit
Target Protein:
GCAP3
Immunogen Range:
141-209/209
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
9626
Source:
KLH conjugated synthetic peptide derived from human GCAP3
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
The intracellular stimulation of guanylate cyclase (GC) by calcium, a key event in the recovery of the dark state of rod photoreceptors after exposure to light, is mediated by guanylate cyclase-activating proteins (GCAP). GCAPs are calcium-binding proteins belonging to the calmodulin superfamily and are specifically expressed in retina. GCAP3 (Guanylyl cyclase-activating protein 3), also known as GUCA1C (Guanylate cyclase activator 1C), is a 209 amino acid EF-hand calcium binding protein that is activated by the decrease in calcium from the absorption of light by rhodopsin. Activation of GCAP3 leads to stimulation of guanylate cyclase 1 and 2 (GC1 and GC2), which increases cGMP concentration. Calcium sensitive regulation of GC is essential in recovery of the rod receptor dark state following light exposure. There are two isoforms of GCAP3 that are produced as a result of alternative splicing events.
Conjugation:
AbBy Fluor® 350
Excitation/ Emission:
346nm/442nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
24
Human
For research use only. Not intended for diagnostic or therapeutic use.