bs-1329R-Cy7 [Conjugated Primary Antibody]
ZO-1 Polyclonal Antibody, Cy7 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ZO-1

Immunogen Range: 1551-1702/1733


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7082

Swiss Prot: Q07157

Source: KLH conjugated synthetic peptide derived from human ZO-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 191


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig

Predicted Cross Reactive Species: Dog
Cow
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926.Read more>>
  • Ruan, Zheng, et al. "Chlorogenic Acid Decreases Intestinal Permeability and Increases Expression of Intestinal Tight Junction Proteins in Weaned Rats Challenged with LPS." PLoS ONE 9.6 (2014): e97815.Read more>>
  • Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512.Read more>>
  • Ruan, Zheng, et al. "Chlorogenic acid enhances intestinal barrier by decreasing MLCK expression and promoting dynamic distribution of tight junction proteins in colitic rats." Journal of Functional Foods 26 (2016): 698-708.Read more>>
  • Kulhankova, Katarina, et al. "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." Infection and immunity (2017): IAI-00848.Read more>>
  • Wang et al. Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line. (2014) PLoS.One. 9:e110916Read more>>
  • Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299Read more>>
  • Mohlin et al. A model to study complement involvement in experimental retinal degeneration. (2018) Ups.J.Med.Sci. 123:28-42Read more>>
  • Zhuang S et al. Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs.(2019)Life Sci. Life Sci. Jan 1;216:168-175. Read more>>
  • Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715.Read more>>
  • Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.Read more>>
  • Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.Read more>>
  • Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
  • Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398. Read more>>
  • Foertsch S et al. Sensory contact to the stressor prevents recovery from structural and functional heart damagefollowing psychosocial trauma. Brain Behav Immun. 2019 May 11. Read more>>
  • Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044.Read more>>
  • Song M et al. Chenodeoxycholic acid (CDCA) protects against the LPS-induced impairment of intestinal epithelial barrier function via FXR-MLCK pathway. J Agric Food Chem. 2019 Aug 5.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Guo Y et al. Impaired intestinal barrier function in a mouse model of hyperuricemia. Mol Med Rep. 2019 Aug 12. Read more>>
  • Ho SW et al. Effects of supplementation of citrulline and Lactobacillus helveticus ASCC 511 on intestinal epithelial cell integrity. Journal of Functional Foods,2019 103571. Read more>>
  • Zhang W et al. Role of EGFR/ErbB2 and PI3K/AKT/e-NOS in Lycium barbarum polysaccharides Ameliorating Endothelial Dysfunction Induced by Oxidative Stress. Am J Chin Med. 2019 Oct 23:1-17. Read more>>
  • Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. Read more>>
  • Wang X et al. LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells. International Journal of Biological Sciences. 2020; 16(2): 251-263. Read more>>
  • Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5. Read more>>
  • Bhushan A et al. MICROFLUIDIC DEVICE WITH EXTRACELLULAR MATRIX SUPPORT MEMBRANE. US Patent App. 16/270,376, 2019Read more>>
  • Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
  • Su H et al. cis 9, trans 11, but not trans 10, cis 12 CLA isomer, impairs intestinal epithelial barrier function in IPEC-J2 cells and mice through activation of GPR120-[Ca2+]i and the MLCK signaling pathway. Food Funct. 2020 Apr 30;11(4):3657-3667. Read more>>
  • Wang Y et al. Ma Xing Shi Gan Decoction Protects against PM2.5-Induced Lung Injury through Suppression of Epithelial-to-Mesenchymal Transition (EMT) and Epithelial Barrier Disruption. Evid Based Complement Alternat Med. 2020 Jun 17;2020:7176589.Read more>>
  • Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene. 2020 Oct 30;759:144999.Read more>>
  • Sebastian G et al. Lythrum salicaria Ellagitannins Stimulate IPEC-J2 Cells Monolayer Formation and Inhibit Enteropathogenic Escherichia coli Growth and AdhesionJ Nat Prod.2020 Dec 24;83(12):3614-3622.Read more>>
  • Y D et al. ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by HyperoxiaBiomed Res Int.2020 Dec 26;2020:8819195.Read more>>
  • Yan Luo. et al. Effects of norepinephrine on colonic tight junction protein expression during heat stress. Exp Ther Med. 2021 May;21(5):1-9Read more>>
  • Ning Xu. et al. Protective effect and mechanism of rebamipide on NSAIDs associated small bowel injury. Int Immunopharmacol. 2021 Jan;90:107136Read more>>
  • Zhang Boxun. et al. The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Evid-Based Compl Alt. 2020;2020:4568629Read more>>
  • Yaqi Chang. et al. Glucagon\like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032Read more>>
  • Paola Pietrangeli. et al. Lathyrus sativus diamine oxidase reduces Clostridium difficile toxin A\induced toxicity in Caco\2 cells by rescuing RhoA\GTPase and inhibiting pp38\MAPK/NF\B/HIF\1 activation. Phytother Res. 2021 Jan;35(1):415-423Read more>>
  • Yanting Hou. et al. Solid-in-oil nanodispersions as a novel delivery system to improve the oral bioavailability of bisphosphate, risedronate sodium. Eur J Pharm Sci. 2020 Dec;155:105521Read more>>
  • Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379Read more>>
  • Jiali Yuan. et al. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1/miR-29b/MMPs pathway in human epidermal keratinocytes. Part Fibre Toxicol. 2021 Dec;18(1):1-22Read more>>
  • Wanfa Dong. et al. Oral delivery of staphylococcal nuclease ameliorates DSS induced ulcerative colitis in mice via degrading intestinal neutrophil extracellular traps. Ecotox Environ Safe. 2021 Jun;215:112161Read more>>
  • Masayoshi Ko. et al. Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Dis Model Mech. 2021 Mar;14(3)Read more>>
  • Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against -Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816Read more>>
  • Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184Read more>>
  • Yuting Qin. et al. Colonic mucus-accumulating tungsten oxide nanoparticles improve the colitis therapy by targeting Enterobacteriaceae. Nano Today. 2021 Aug;39:101234Read more>>
  • Jin Zhao. et al. Damage to intestinal barrier integrity in piglets caused by porcine reproductive and respiratory syndrome virus infection. Vet Res. 2021 Dec;52(1):1-14Read more>>
  • Xiao Wang. et al. Oral CoreCShell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES