bs-1329R [Primary Antibody]
ZO-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ZO-1

Immunogen Range: 1551-1702/1733


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7082

Swiss Prot: Q07157

Source: KLH conjugated synthetic peptide derived from human ZO-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 191


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Pig
Chicken
Goat
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926.Read more>>
  • Ruan, Zheng, et al. "Chlorogenic Acid Decreases Intestinal Permeability and Increases Expression of Intestinal Tight Junction Proteins in Weaned Rats Challenged with LPS." PLoS ONE 9.6 (2014): e97815.Read more>>
  • Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512.Read more>>
  • Ruan, Zheng, et al. "Chlorogenic acid enhances intestinal barrier by decreasing MLCK expression and promoting dynamic distribution of tight junction proteins in colitic rats." Journal of Functional Foods 26 (2016): 698-708.Read more>>
  • Kulhankova, Katarina, et al. "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." Infection and immunity (2017): IAI-00848.Read more>>
  • Wang et al. Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line. (2014) PLoS.One. 9:e110916Read more>>
  • Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299Read more>>
  • Mohlin et al. A model to study complement involvement in experimental retinal degeneration. (2018) Ups.J.Med.Sci. 123:28-42Read more>>
  • Zhuang S et al. Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs.(2019)Life Sci. Life Sci. Jan 1;216:168-175. Read more>>
  • Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715.Read more>>
  • Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.Read more>>
  • Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.Read more>>
  • Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
  • Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398. Read more>>
  • Foertsch S et al. Sensory contact to the stressor prevents recovery from structural and functional heart damagefollowing psychosocial trauma. Brain Behav Immun. 2019 May 11. Read more>>
  • Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044.Read more>>
  • Song M et al. Chenodeoxycholic acid (CDCA) protects against the LPS-induced impairment of intestinal epithelial barrier function via FXR-MLCK pathway. J Agric Food Chem. 2019 Aug 5.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.Read more>>
  • Guo Y et al. Impaired intestinal barrier function in a mouse model of hyperuricemia. Mol Med Rep. 2019 Aug 12. Read more>>
  • Ho SW et al. Effects of supplementation of citrulline and Lactobacillus helveticus ASCC 511 on intestinal epithelial cell integrity. Journal of Functional Foods,2019 103571. Read more>>
  • Zhang W et al. Role of EGFR/ErbB2 and PI3K/AKT/e-NOS in Lycium barbarum polysaccharides Ameliorating Endothelial Dysfunction Induced by Oxidative Stress. Am J Chin Med. 2019 Oct 23:1-17. Read more>>
  • Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. Read more>>
  • Wang X et al. LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells. International Journal of Biological Sciences. 2020; 16(2): 251-263. Read more>>
  • Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5. Read more>>
  • Bhushan A et al. MICROFLUIDIC DEVICE WITH EXTRACELLULAR MATRIX SUPPORT MEMBRANE. US Patent App. 16/270,376, 2019Read more>>
  • Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
  • Su H et al. cis 9, trans 11, but not trans 10, cis 12 CLA isomer, impairs intestinal epithelial barrier function in IPEC-J2 cells and mice through activation of GPR120-[Ca2+]i and the MLCK signaling pathway. Food Funct. 2020 Apr 30;11(4):3657-3667. Read more>>
  • Wang Y et al. Ma Xing Shi Gan Decoction Protects against PM2.5-Induced Lung Injury through Suppression of Epithelial-to-Mesenchymal Transition (EMT) and Epithelial Barrier Disruption. Evid Based Complement Alternat Med. 2020 Jun 17;2020:7176589.Read more>>
  • Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene. 2020 Oct 30;759:144999.Read more>>
  • Sebastian G et al. Lythrum salicaria Ellagitannins Stimulate IPEC-J2 Cells Monolayer Formation and Inhibit Enteropathogenic Escherichia coli Growth and AdhesionJ Nat Prod.2020 Dec 24;83(12):3614-3622.Read more>>
  • Y D et al. ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by HyperoxiaBiomed Res Int.2020 Dec 26;2020:8819195.Read more>>
  • Yan Luo. et al. Effects of norepinephrine on colonic tight junction protein expression during heat stress. Exp Ther Med. 2021 May;21(5):1-9Read more>>
  • Ning Xu. et al. Protective effect and mechanism of rebamipide on NSAIDs associated small bowel injury. Int Immunopharmacol. 2021 Jan;90:107136Read more>>
  • Zhang Boxun. et al. The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Evid-Based Compl Alt. 2020;2020:4568629Read more>>
  • Yaqi Chang. et al. Glucagon\like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032Read more>>
  • Paola Pietrangeli. et al. Lathyrus sativus diamine oxidase reduces Clostridium difficile toxin A\induced toxicity in Caco\2 cells by rescuing RhoA\GTPase and inhibiting pp38\MAPK/NF\B/HIF\1 activation. Phytother Res. 2021 Jan;35(1):415-423Read more>>
  • Yanting Hou. et al. Solid-in-oil nanodispersions as a novel delivery system to improve the oral bioavailability of bisphosphate, risedronate sodium. Eur J Pharm Sci. 2020 Dec;155:105521Read more>>
  • Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379Read more>>
  • Jiali Yuan. et al. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1/miR-29b/MMPs pathway in human epidermal keratinocytes. Part Fibre Toxicol. 2021 Dec;18(1):1-22Read more>>
  • Wanfa Dong. et al. Oral delivery of staphylococcal nuclease ameliorates DSS induced ulcerative colitis in mice via degrading intestinal neutrophil extracellular traps. Ecotox Environ Safe. 2021 Jun;215:112161Read more>>
  • Masayoshi Ko. et al. Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Dis Model Mech. 2021 Mar;14(3)Read more>>
  • Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against -Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816Read more>>
  • Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184Read more>>
  • Yuting Qin. et al. Colonic mucus-accumulating tungsten oxide nanoparticles improve the colitis therapy by targeting Enterobacteriaceae. Nano Today. 2021 Aug;39:101234Read more>>
  • Jin Zhao. et al. Damage to intestinal barrier integrity in piglets caused by porcine reproductive and respiratory syndrome virus infection. Vet Res. 2021 Dec;52(1):1-14Read more>>
  • Xiao Wang. et al. Oral CoreCShell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining


Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.


Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.


293T cells probed with ZO-1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-1329R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).


293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Human HUVEC cell lysates; Lane 2: Human A431 cell lysates probed with ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 polyclonal antibody, unconjugated (bs-1329R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.