DATASHEET
Host:
Rabbit
Target Protein:
GAS2
Immunogen Range:
141-240/313
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
2620
Source:
KLH conjugated synthetic peptide derived from human GAS2
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Gas2 is a 313 amino acid protein encoded by the human gene GAS2. Gas2 is thought to play a role in apoptosis by acting as a cell death substrate for caspases. Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 and caspase-7) at Asparagine 278 during apoptosis. The cleaved form resulting from this dramatically induces the rearrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells. Gas2 is believed to also be involved in the membrane ruffling process. During the G0-G1 transition phase Gas2 can be found phosphorylated on its serine residues. Gas2 is a cytoskeleton and peripheral membrane protein that co-localizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Gas2 is mainly membrane-associated but when hyperphosphorylated it will accumulate at membrane ruffles. Gas2 is specifically expressed at growth arrest and is ubiquitously expressed with highest levels found in liver, lung and kidney. There is no evidence, however, of Gas2 expression in spleen.
Conjugation:
Cy5.5
Excitation/ Emission:
675nm/694nm
Size:
100ul
Concentration:
1ug/ul
Applications:
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
35
Predicted Cross Reactive Species:
Human
Mouse
Rat
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.