bs-13253R [Primary Antibody]
G6PC3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: G6PC3

Immunogen Range: 201-300/346


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 92579

Swiss Prot: Q9BUM1

Source: KLH conjugated synthetic peptide derived from human G6PC3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glucose-6-phosphatase (G6Pase), is a multicomponent enzyme system that hydrolyzes glucose-6-phosphate (G6P) in the final step of gluconeogenesis and gluconeolysis. G6Pase localizes to the endoplasmic reticulum, and while liver, kidney, and intestine are the only tissues that express the first identified isoform, G6Pase-Alpha, a second form, designated G6Pase-Beta, contributes to blood glucose homeostasis in a wider range of tissues. G6Pase-Beta, also known as SCN4, UGRP or G6PC3 (glucose 6 phosphatase, catalytic, 3), is a 346 amino acid endoplasmic reticulum multi-pass membrane protein that is involved in carbohydrate biosynthesis and the gluconeogenesis pathway. Inhibited by vanadate, G6Pase-Beta hydrolyzes GP6 to glucose in the endoplasmic reticulum. Due to its necessary involvement in normal glucose metabolism, G6Pase-Beta may play an integral role in diabetes and glycogen storage diseases (GSDs).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Acevedo C et al. Possible ATP trafficking by ATP-shuttles in the olfactory cilia and glucose transfer across the olfactory mucosa. FEBS Lett. 2019 Feb 22.Read more>>
  • Jiang, Bin. et al. Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery. Obes Surg. 2022 Jan;:1-11Read more>>
  • Jiahui Li. et al. Dietary iron modulates hepatic glucose homeostasis via regulating gluconeogenesis. J NUTR BIOCHEM. 2022 Nov;109:109104Read more>>
  • Naomi Nishio. et al. Hen egg only diets support healthy aging in adult mice. J ANIM PHYSIOL AN N. 2023 JaRead more>>
  • Yue Pang. et al. Expression Regulation of Gluconeogenesis Related Genes in Ovine Skeletal Muscle Cells. FRONT BIOSCI-LANDMRK. 2024 Jun;29(6):237Read more>>
VALIDATION IMAGES

Lane 1: Mouse Skeletal muscle lysates; Lane 2: Rat Skeletal muscle lysates probed with G6PC3 Polyclonal Antibody, Unconjugated (bs-13253R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.