bs-13195R [Primary Antibody]
Folate Receptor 4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Folate Receptor 4

Immunogen Range: 175-225/250


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 390243

Swiss Prot: A6ND01

Source: KLH conjugated synthetic peptide derived from human Folate Receptor 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Folate receptor 4 is a highly expressed cell surface marker of regulatory T cells. Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. This receptor does not bind folate.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiong Lai. et al. Participation of WD repeat-containing protein 54 (WDR54) in rat sperm-oocyte fusion through interaction with both IZUMO1 and JUNO. THERIOGENOLOGY. 2024 Jan;214:286Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human stomach carcinoma labeled withAnti-Folate Receptor 4 Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\n


Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Folate Receptor 4) Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Folate Receptor 4) Polyclonal Antibody, Unconjugated (bs-13195R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.