Host: Rabbit
Target Protein: FAM134B
Specificity: There is a chance that this protein will cross-react with MSL2 based on a 73% non-sequential sequence similarity.
Immunogen Range: 210-310/497
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54463
Swiss Prot: Q9H6L5
Source: KLH conjugated synthetic peptide derived from human FAM134B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yiyuan Yang. et al. Apelin-13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin-1/P2X7 axis and FAM134B-dependent reticulophagy. 2022 Feb 06Read more>>
Lane 1: Mouse testis lysates; Lane 2: Hela lysates probed with FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart labeled with Anti-FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134B Polyclonal Antibody, Unconjugated (bs-13136R) at 1:400 overnight at 4°C, DAB staining.
U-937 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FAM134B Polyclonal Antibody(bs-13136R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).