bs-13120R [Primary Antibody]
EXOSC10/PMSCL2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EXOSC10/PMSCL2

Immunogen Range: 41-140/885


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5394

Source: KLH conjugated synthetic peptide derived from human EXOSC10/PMSCL2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The exosome is a multi-subunit complex composed of several highly conserved proteins, some of which are 3? to 5? exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3? region. EXOSC10, also known as PMSCL, PMSCL2, p2, p3, p4, RRP6, Rrp6p, PM-Scl, or PM/Scl-100, is an 885 amino acid protein that contains one HRDC domain and one 3?-5? enonuclease domain. Localized to both the cytoplasm and the nucleus, EXOSC10 is part of the post-splicing exosome complex and is involved in mRNA surveillance, mRNA nuclear export and nonsense-mediated decay of mRNAs containing premature stop codons. against EXOSC10 have been found in patients with scleroderma and/or polymyositis (chronic diseases of the skin and muscle, respectively), suggesting that EXOSC10 may be involved in the pathogenesis of these diseases. Two isoforms of EXOSC10 exist due to alternative splicing events.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 101


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human skin labeled with Anti-EXOSC10/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\n


Formalin-fixed and paraffin embedded mouse lymph node labeled with Anti-EXOSC10/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\n


U937 cell lysates probed with EXOSC10/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with EXOSC10/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 overnight at 4°C, DAB staining.