Host: Rabbit
Target Protein: ETL/ELTD1
Immunogen Range: 301-400/690
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 64123
Swiss Prot: Q9HBW9
Source: KLH conjugated synthetic peptide derived from human ETL/ELTD1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 76
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Zalles M et al. Optimized monoclonal antibody treatment against ELTD1 for GBM in a G55 xenograft mouse model. J Cell Mol Med. 2019 Dec 21.Read more>>
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ETL/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tETL/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C and DAPI staining.