bs-1310R-BF647 [Conjugated Primary Antibody]
Integrin Alpha V Polyclonal Antibody, AbBy Fluor® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Integrin Alpha V

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3685

Swiss Prot: P06756

Source: KLH conjugated synthetic peptide derived from human Integrin Alpha V

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Conjugation: AbBy Fluor® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mondal, Goutam, Sugata Barui, and Arabinda Chaudhuri. "The relationship between the cyclic-RGDfK ligand and αvβ3 integrin receptor." Biomaterials (2013).Read more>>
  • Jiang, Lei, et al. "64Cu-Labeled Divalent Cystine Knot Peptide for Imaging Carotid Atherosclerotic Plaques." Journal of Nuclear Medicine (2015): jnumed-115.Read more>>
  • Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015).Read more>>
  • Nande, Rounak Paramjeet, "Investigation Of Ultrasound Targeted Microbubbles As A Therapeutic Gene Delivery System For Prostate Cancer" (2015). Theses, Dissertations and Capstones. Paper 915.Read more>>
  • Chen, Li, and David R. Brigstock. "Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC‐derived exosomes." FEBS letters (2016).Read more>>
  • Bian, Qin, et al. "Mechanosignaling activation of TGFβ maintains intervertebral disc homeostasis." Bone Research 5 (2017): 17008Read more>>
  • Li, Yin, Lin Xiong, and Jianping Gong. "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." Am J Transl Res 9.6 (2017): 2865-2877.Read more>>
  • Chen, Li, et al. "Pathways of production and delivery of hepatocyte exosomes." Journal of Cell Communication and Signaling (2017): 1-15.Read more>>
  • Zhang, Yingying, et al. "Synthesis and Bioevaluation of Iodine-131 Directly Labeled Cyclic RGD-PEGylated Gold Nanorods for Tumor-Targeted Imaging." Contrast Media & Molecular Imaging 2017 (2017).Read more>>
  • Li et al. Effects of Cationic Microbubble Carrying CD/TK Double Suicide Gene and αVβ3 Integrin Antibody in Human Hepatocellular Carcinoma HepG2 Cells. (2016) PLoS.On. 11:e0158592Read more>>
  • Ma et al. Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis. (2017) Sci.Rep. 7:4978Read more>>
  • Shimojo et al. Tenascin-C may accelerate cardiac fibrosis by activating macrophages via the integrin αVβ3/nuclear factor-κB/interleukin-6 axis. (2015) Hypertension. 66:757-66Read more>>
  • Yan et al. (99m)Tc-3P-RGD2 molecular imaging targeting integrin α v β 3 in head and neck squamous cancer xenograft. (2015) J.Radioanal.Nucl.Chem. 304:1171-1177Read more>>
  • Jiang L et al. Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Mol Pharm. 2019 Mar 4;16(3):1350-1357. Read more>>
  • Wong L et al. Substrate Stiffness Directs Diverging Vascular Fates. Acta Biomater. 2019 Jul 19. pii: S1742-7061(19)30519-7.Read more>>
  • Ozlem Ozen Karakus et al. Dual Targeting of Norepinephrine Transporter (NET) Function and Thyrointegrin v3 Receptors in the Treatment of Neuroblastoma. J Med Chem. 2020 Jul 23;63(14):7653-7662.Read more>>
  • Jaya Aseervathamet al. Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci. 2020 Jul 2;21(13):4734.Read more>>
  • Park CR et al. Conjugation of arginylglycylaspartic acid to Human serum albumin decreases the tumor-targeting effect of albumin by hindering its secreted protein acidic and rich in cysteine-mediated accumulation in tumors. Am J Transl Res. 2020 Jun 15;12(6):2488-2498.Read more>>
  • Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
  • Yusuke Mochizuki. et al. Telomerase-specific oncolytic immunotherapy for promoting efficacy of PD-1 blockade in osteosarcoma. 2020 Nov 05Read more>>
  • Gehua Zhen. et al. Mechanical stress determines the configuration of TGF activation in articular cartilage. Nat Commun. 2021 Mar;12(1):1-16Read more>>
  • Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
  • Yun Long. et al. GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation. Front Physiol. 2021; 12: 674924Read more>>
  • Ozlem Ozen Karakus. et al. Discovery of dual targeting PEGylated BG-P1600-TAT to norepinephrine transporter (NET) and thyrointegrin v3 in the treatment of neuroblastoma. Bioorgan Med Chem. 2021 Aug;43:116278Read more>>
  • Kuo Zhang. et al. A Monotargeting Peptidic Network Antibody Inhibits More Receptors for Anti-Angiogenesis. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Dan Lin. et al. iRGD-modified exosomes effectively deliver CPT1A siRNA to colon cancer cells, reversing oxaliplatin resistance by regulating fatty acid oxidation. 2021 Jul 02Read more>>
  • Kosuke Mitsuyuki. et al. Evaluation of Integrin v3 Expression in Murine Xenograft Models: [68Ga]Ga-DOTA-C(RGDfK) PET Study with Immunohistochemical Confirmation. Diagnostics. 2021 Jul;11(7):1295Read more>>
  • Ting Li. et al. Albumin Fusion at the N-Terminus or C-Terminus of HM-3 Leads to Improved Pharmacokinetics and Bioactivities. Biomedicines. 2021 Sep;9(9):1084Read more>>
  • Zhang, Li-xue. et al. Expression of SGLT1 in the Mouse Endometrial Epithelium and its Role in Early Embryonic Development and Implantation. 2021 Aug 3Read more>>
  • Rui-Qin Yang. et al. Development and Preclinical Evaluation of a Near-Infrared Fluorescence Probe Based on Tailored Hepatitis B Core Particles for Imaging-Guided Surgery in Breast Cancer. Int J Nanomed. 2022; 17: 1343136Read more>>
  • Wang, Congcong. et al. Engineering a HEK-293T exosome-based delivery platform for efficient tumor-targeting chemotherapy/internal irradiation combination therapy. J NANOBIOTECHNOL. 2022 Dec;20(1):1-17Read more>>
  • Hasan Al Faruque. et al. Enhanced effect of autologous EVs delivering paclitaxel in pancreatic cancer. J CONTROL RELEASE. 2022 Jul;347:33Read more>>
  • Andrew S. Choi. et al. Can targeted nanoparticles distinguish cancer metastasis from inflammation?. J CONTROL RELEASE. 2023 ApRead more>>
  • Llancalahuen Felipe M.. et al. 1-Adrenergic Stimulation Increases Platelet Adhesion to Endothelial Cells Mediated by TRPC6. ADV EXP MED BIOL. 2023 Apr 25Read more>>
  • Khalil H. Alshanqiti. et al. Irisin Induces Apoptosis in Metastatic Prostate Cancer Cells and Inhibits Tumor Growth In Vivo. CANCERS. 2023 Jan;15(15):4Read more>>
  • Hui Zhang. et al. A peptidic network antibody inhibits both angiogenesis and inflammatory response. J CONTROL RELEASE. 2023 Oct;362:715Read more>>
  • Nofi Colleen P. et al. A novel opsonic eCIRP inhibitor for lethal sepsis. J LEUKOCYTE BIOL. 2023 SepRead more>>
  • Hans-Georg Lerchen. et al. Discovery of VIP236, an v3-Targeted Small-MoleculeCDrug Conjugate with Neutrophil Elastase-Mediated Activation of 7-Ethyl Camptothecin Payload for Treatment of Solid Tumors. CANCERS. 2023 Jan;15(17):4381Read more>>
  • Guo Xiaoye. et al. The influence of a modified p53 C-terminal peptide by using a tumor-targeting sequence on cellular apoptosis and tumor treatment. APOPTOSIS. 2023 Dec;:1-17Read more>>
  • Xiangwu Geng. et al. Biomimetically ordered ultralong hydroxyapatite nanowires-based hierarchical hydrogel scaffold with osteoimmunomodulatory and osteogenesis abilities for augmenting bone regeneration. CHEM ENG J. 2024 May;488:151136Read more>>
  • Geng Xiangwu. et al. Integrin V3 antagonist-c(RGDyk) peptide attenuates the progression of ossification of the posterior longitudinal ligament by inhibiting osteogenesis and angiogenesis. MOL MED. 2024 Dec;30(1):1-15Read more>>
  • Elif Seda zdemir. et al. The effect of folic acid supplementation and folate deficiency on embryo implantation. REPROD BIOL. 2024 Sep;24:100895Read more>>
  • Xiaoyin Guo. et al. Mechanism underlying the role of integrin 31 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles. SCI TOTAL ENVIRON. 2024 Oct;947:174535Read more>>
  • Sidorenko Valeria. et al. Targeting vascular disrupting agent-treated tumor microenvironment with tissue-penetrating nanotherapy. SCI REP-UK. 2024 Jul;14(1):1-16Read more>>
  • ockhaus Katrin. et al. Integrin 2 is an early marker for osteoclast differentiation that contributes to key steps in osteoclastogenesis. FRONT CELL DEV BIOL. 2024 Aug;12Read more>>
VALIDATION IMAGES