bs-1309R [Primary Antibody]
Syndecan 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD138/Syndecan 1

Immunogen Range: 201-310/310


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6382

Swiss Prot: P18827

Source: KLH conjugated synthetic peptide derived from human CD138

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse
Rat
Bovine
Cow

Predicted Cross Reactive Species: Dog
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Miyatani, Kozo, et al. "A high number of IgG4-positive cells in gastric cancer tissue is associated with tumor progression and poor prognosis." Virchows Archiv (2016): 1-9.Read more>>
  • Chen, Song, et al. "Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis." Journal of Histochemistry & Cytochemistry (2017): 0022155417692536.Read more>>
  • Hambruch, N., et al. "Bovine placentomal heparanase and syndecan expression is related to placental maturation." Placenta.Read more>>
  • Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
  • Linlin Yanget al. CAMKII is a targetable driver of multiple myeloma through CaMKII/ Stat3 axis. Aging (Albany NY). 2020 Jul 13;12(13):13668-13683.Read more>>
  • Jiang Yufei. et al. Osteoprotegerin interacts with syndecan-1 to promote human endometrial stromal decidualization by decreasing Akt phosphorylation. Hum Reprod. 2020 Nov;35(11):2439-2453Read more>>
  • Haibo Xie. et al. Endothelin-1 down-regulated vascular endothelial growth factor A is involved in trichloroethene-induced kidney injury:. TOXICOL IND HEALTH. 2022;38(5):287-298Read more>>
  • Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringers solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331Read more>>
  • You Shuang. et al. Recombinant humanized collagen remodels endometrial immune microenvironment of chronic endometritis through macrophage immunomodulation. REGEN BIOMATER. 2023 ApRead more>>
  • Zhenjie Wang. et al. Effect of sodium bicarbonate Ringer's solution on lung injury in rats with traumatic hemorrhagic shock. J BIOCHEM MOL TOXIC. 2023 Dec;:e23608Read more>>
  • iebele Abel. et al. The Degree of Aortic Occlusion in the Setting of Trauma Alters the Extent of Acute Kidney Injury Associated with Mitochondrial Preservation. AM J PHYSIOL-RENAL. 2024 Mar;(Articles in Press)Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti CD138/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 human colon carcinoma lysates probed with Anti- CD138/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 34kD.Observed band 34D, 92kD


Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Syndecan 1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary and for 20 minutes and DAB staining.


Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human HeLa cell lysates probed with Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.